Phosphoinositide-3-OH-kinase inhibitor LY294002 prevents activation of Ancylostoma caninum and Ancylostoma ceylanicum third-stage infective larvae

International Journal for Parasitology
Andrea Brand, John M Hawdon

Abstract

The developmentally arrested hookworm infective larva resumes development only after encountering specific host-mediated cues during invasion. These cues activate a signaling pathway that culminates in the resumption of development. In Ancylostoma caninum, activation is characterised by the resumption of feeding and the release of excretory/secretory products required for infection. The dauer stage of the free-living nematode Caenorhabditis elegans is a developmentally arrested stage analogous to the hookworm infective larva. Dauer larvae exit developmental arrest in response to environmental cues that indicate favorable conditions for reproduction and growth. Because of the similarity between dauer recovery and activation, exit from dauer provides a model for hookworm larval activation. An insulin-signaling pathway has been implicated in controlling exit from developmental arrest in both C. elegans dauers and A. caninum larvae. To further investigate the role of insulin signaling in hookworm larval activation, the phosphatidylinositol-3-OH kinase inhibitor LY294002 was tested for its effect on in vitro activation using the resumption of feeding as a marker for activation. LY294002 prevented feeding in A. caninum infective larv...Continue Reading

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Citations

Jun 28, 2005·Molecular and Biochemical Parasitology·Jennifer M MoserGreg Gibson
Dec 21, 2012·Parasitology·Juan E Palomares-RiusVivian C Blok
Apr 28, 2005·BMC Genomics·Makedonka MitrevaRichard K Wilson
Jun 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zhu WangDavid J Mangelsdorf
Feb 5, 2016·Parasites & Vectors·Namitha MohandasRobin B Gasser
Mar 1, 2011·Veterinary Parasitology·Dilyan I DryanovskiJohn M Hawdon
Oct 22, 2008·International Journal for Parasitology·Xin GaoJohn M Hawdon
Aug 25, 2015·Molecular and Biochemical Parasitology·Verena GelmedinJohn M Hawdon
Sep 2, 2005·International Journal for Parasitology·Andrea M BrandJohn M Hawdon
Dec 22, 2010·International Journal for Parasitology·Joseph KreppJohn M Hawdon
Jul 27, 2010·International Journal for Parasitology·Stanley Ching-Cheng HuangMurray E Selkirk
Oct 8, 2013·International Journal for Parasitology·Matt Crook
Jun 26, 2008·The Journal of Parasitology·Beverly J WolpertJohn M Hawdon
Dec 9, 2016·Nature Reviews. Disease Primers·Alex LoukasJeffrey M Bethony

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