Phospholamban phosphorylation sites enhance the recovery of intracellular Ca2+ after perfusion arrest in isolated, perfused mouse heart

Cardiovascular Research
Carlos A ValverdeAlicia Mattiazzi

Abstract

To investigate the importance of the phosphorylation of Ser16 and Thr17 sites of phospholamban (PLN) on intracellular Ca2+ (Cai2+) handling and contractile recovery of the stunned myocardium. Cai2+ (Rhod-2, pulsed local-field fluorescence microscopy) and contractility (isovolumic left ventricular developed pressure, LVDP) were simultaneously measured in Langendorff perfused hearts from transgenic mice expressing either intact PLN (PLN-WT) or PLN with both phosphorylation sites mutated to Ala (PLN-DM), subjected to 12 min of global ischemia followed by a reperfusion period of 30 min. Pre-ischemic values of Cai2+ and LVDP were similar in both groups. In PLN-WT, a transient increase in Thr17 phosphorylation at early reperfusion preceded a recovery of Ca2+ transient amplitude, virtually completed by the end of reperfusion. LVDP at 30 min reperfusion was 67.9+/-7.6% of pre-ischemic values, n=14. In contrast, in PLN-DM, there was a poor recovery of Cai2+ transient amplitude and LVDP was significantly lower (28.3+/-6.7%, n=11, 30 min reperfusion) than in PLN-WT hearts. Although myofilament Ca2+ responsiveness and troponin I (TnI) degradation did not differ between groups, the episodes of mechanical alternans, typical of Cai2+ overload...Continue Reading

Citations

Nov 19, 2009·Cardiovascular Research·Carlos A ValverdeAriel L Escobar
Dec 27, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Marcela FerreiroAriel L Escobar
May 15, 2013·PloS One·Paula D Mullins, Vladimir E Bondarenko
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Jan 16, 2007·Cardiovascular Research·Alicia MattiazziCecilia Mundiña-Weilenmann
Jan 16, 2007·Cardiovascular Research·Martin Vila-PetroffAlicia Mattiazzi
Nov 6, 2013·Biochimica Et Biophysica Acta·Martin W Berchtold, Antonio Villalobo
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Dec 23, 2009·Journal of Molecular and Cellular Cardiology·Martín Vila-PetroffAlicia Mattiazzi
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