PMID: 11916149Mar 28, 2002Paper

Phospholipase A2 from Trypanosoma brucei gambiense and Trypanosoma brucei brucei: inhibition by organotins

Journal of Enzyme Inhibition
M N ShuaibuA J Nok

Abstract

Activity and kinetics of phospholipase A2 (PLA2) from Trypanosoma brucei gambiense (Wellcome strain) and Trypanosoma brucei brucei (GUTat 3.1) were examined using two different fluorescent substrates. The activity in the supernatants of sonicated parasites was Ca2+-independent, strongly stimulated by Triton X-100 with optimum activity at 37 degrees C and pH 6.5-8.5. To encourage a possible interaction between the parasite enzyme and organotin compounds, fatty acid derivatives of dibutyltin dichloride were synthesized and evaluated as potential inhibitors of PLA2. The enzyme from the two-trypanosome species differ with respect to kinetic parameters and are noncompetitively inhibited by the organotin compounds. The Michaelis constant (KM) for PLA2 from T. b. brucei is 63.87 and 30.90 microM while for T. b. gambiense it is 119.64 and 32.91 microM for the substrates 1,2-bis-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine (PBGPC) and 2-(12-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine (NBDC12-HPC), respectively.

References

Dec 1, 1976·Experimental Parasitology·W J Herbert, W H Lumsden
Sep 1, 1991·The Journal of protozoology·L D Saffer, J D Schwartzman
Dec 1, 1970·Experimental Parasitology·S M Lanham, D G Godfrey
Jun 29, 1984·Biochemical and Biophysical Research Communications·M C Connelly, F Kierszenbaum
Feb 1, 1981·Molecular and Biochemical Parasitology·P N HambreyI R Tizard
Jan 1, 1996·Parasitology Research·A J NokP C Onyenekwe
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G A Cross
May 6, 1998·Parasitology Research·J Vargas-VillarrealA Alagón-Cano
Sep 11, 1998·Archives of Environmental Contamination and Toxicology·A Pain, J J Cooney
May 4, 1999·Memórias do Instituto Oswaldo Cruz·S L Croft
Jan 19, 2000·Parasitology Today·S V Barrett, M P Barrett
Apr 1, 2000·Parasite : Journal De La Société Française De Parasitologie·M N ShuaibuA J Nok
Oct 12, 2000·International Journal for Parasitology·S CassaingH Chap
Jan 1, 1994·Parasitology Today·H Hirumi, K Hirumi
Aug 1, 1989·Parasitology Today·A Mellors, A Samad
May 1, 1993·Parasitology Today·C J Bacchi

❮ Previous
Next ❯

Citations

Jul 15, 2003·Clinical Microbiology Reviews·Mairi C NoverrGary B Huffnagle
Feb 24, 2004·Infection and Immunity·Kirk J Lubick, Donald E Burgess
May 29, 2007·Trends in Parasitology·Bruno Kilunga KubataYoshihiro Urade
Jan 24, 2007·Molecular Microbiology·Gregory S Richmond, Terry K Smith
May 24, 2011·Enzyme Research·María Laura BelaunzaránElvira Luisa Durante de Isola
Feb 14, 2013·Parasitology Research·Shankar MukherjeeHerbert B Tanowitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.