PMID: 11332933May 3, 2001Paper

Phospholipase A2 is present in meconium and inhibits the activity of pulmonary surfactant: an in vitro study

Acta Paediatrica
A J SchramaH M Berger

Abstract

Atelectasis, a major contributor to pulmonary dysfunction in meconium aspiration syndrome (MAS), is produced by bronchiolar obstruction and surfactant inactivation. It has been shown that substances in meconium, e.g. fatty acids, inhibit surfactant activity. However, the role of the enzyme phospholipase A2 (PLA2), which hydrolyses surfactant in adult respiratory distress syndrome (ARDS), has not yet been studied. Our objective was to investigate whether PLA2 is present in meconium and inhibits pulmonary surfactant activity in vitro. Therefore, the presence of PLA2 activity in meconium, collected from 10 newborns, was measured by the formation of lysophosphatidylcholine after incubation of meconium with radioactively labelled dipalmitoylphosphatidylcholine. Meconium was fractionated by Sephadex G-100 column chromatography and the fractions were assayed for PLA2 activity. Also, their effect on the surface tension of surfactant (Curosurf) was measured using a pulsating bubble surfactometer (PBS). PLA2 activity was present in all meconium samples. Addition of meconium to surfactant significantly increased surface tension (mean +/- SD: 1.7 +/- 1.6 mN/m to 24.3 +/- 6.7 mN/m, p = 0.0001) and only the addition of the PLA2 containing fr...Continue Reading

Citations

Jun 8, 2013·The Journal of Membrane Biology·Oguz TuncerErcan Kirimi
Dec 17, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·P Kääpä, H Soukka
Dec 17, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·T E MollnesO D Saugstad
Dec 17, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·O D SaugstadC A Drevon
Jan 18, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·A J J SchramaF J Walther
Sep 7, 2004·Pediatric Research·Paal H H LindenskovTom E Mollnes
Jan 7, 2005·Pediatric Research·Paal H H LindenskovOla D Saugstad
Apr 22, 2006·Pediatric Research·Tomi SippolaPekka Kääpä
Sep 5, 2003·Intensive Care Medicine·Anne HilgendorffIrwin Reiss
Jun 9, 2007·American Journal of Respiratory Cell and Molecular Biology·Zhengdong WangRobert H Notter
Apr 6, 2011·European Journal of Pediatrics·Daniela Mokra, Juraj Mokry
Nov 10, 2011·BMC Pediatrics·Daniele De LucaUNKNOWN Study group on Secretory Phospholipase in Paediatrics (SSPP)
Jun 4, 2008·European Journal of Pediatrics·Alexandra J J SchramaFrans J Walther
Dec 19, 2015·Pediatric Research·Jana Kopincova, Andrea Calkovska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved