PMID: 8967783Mar 1, 1996Paper

Phospholipase A2 is secreted by murine keratinocytes after stimulation with IL-1 alpha and TNF-alpha

Archives of Dermatological Research
B C BastianE B Bröcker

Abstract

Phospholipase A2 (PLA2)-catalysed liberation of arachidonic acid is the rate-limiting step in the generation of the lipid mediators prostaglandins and leukotrienes. PLA2 regulation thus represents a pivotal mechanism in the pathogenesis of inflammation. In this study we investigated the effects of TNF alpha and IL-1 alpha on PLA2 activity in cultured murine keratinocytes. Starting 18 h after stimulation, PLA2 activity increased significantly by about 250-320%) in the supernatants and in the cell pellets. This effect was completely inhibited either by preincubation of the cells with dexamethasone 48 h before stimulation or by coincubation with actinomycin D. PLA2 activity detected in the supernatants was blocked by reduction with dithiothreitol, whereas the PLA2 activity in the pellets was dithiothreitol-resistant. We conclude that in murine keratinocytes IL-1 alpha induce de novo synthesis and release of a secretory PLA2 and the induction of a different PLA2 activity in the cytosol. These findings indicate a crucial link between early cytokine effects and the initiation of the lipid mediator cascade in keratinocytes. The observation that PLA2 induction could be completely inhibited by preincubation with dexamethasone allows new...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L L LinJ L Knopf
Mar 1, 1991·Research in Virology·L R BraathenO Strand
Apr 24, 1991·Biochimica Et Biophysica Acta·D K KimK Inoue
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J D ClarkJ L Knopf
Jan 1, 1990·The Journal of Investigative Dermatology·A P Pentland, M G Mahoney
Jan 1, 1988·The Journal of Investigative Dermatology·M BergersP D Mier
Mar 1, 1989·British Journal of Pharmacology·E Solito, L Parente
Oct 16, 1989·Biochemical and Biophysical Research Communications·B Lyons-GiordanoE C Arner
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R M BurchJ Axelrod
Jan 1, 1986·Annual Review of Biochemistry·D J Hanahan
Feb 1, 1987·The Journal of Investigative Dermatology·J Kvedar, L Levine
Jul 1, 1985·The Journal of Investigative Dermatology·H KondohH Kanoh
Dec 4, 1985·Biochimica Et Biophysica Acta·A J AarsmanH van den Bosch
Jun 1, 1984·The British Journal of Dermatology·A VerhagenP D Mier
Apr 15, 1982·The Biochemical Journal·R F Irvine
Jan 1, 1983·The British Journal of Dermatology·S ForsterH J Yardley
Sep 1, 1993·The Journal of Investigative Dermatology·B C BastianE B Bröcker
Feb 1, 1993·The American Journal of Physiology·C H Kang-RotondoA P Pentland
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W G HoeckR A Heller

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Citations

Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Lappas, G E Rice
Jan 1, 1997·Photochemistry and Photobiology·K Maeda, M Naganuma
Apr 20, 2000·The Journal of Investigative Dermatology·E MauryH Chap

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