Dec 18, 1975

Phospholipase A2 isoenzyme from porcine pancreas. Purification and some properties

Biochimica Et Biophysica Acta
F M Van Wezel, G H De Haas

Abstract

Porcine pancreas synthesizes a prephospholipase A2 which occurs in a 5 : 95 ratio compared with the more abundant zymogen of the same enzyme (phosphatide-acylhydrolase; EC 3.1.1.4). These two prephospholipases could be well separated by CM-cellulose chromatography. Both the active and the zymogen form of the isoenzyme were isolated and purified. The activation peptides of both prephospholipases appeared to be identical, while the active enzymes showed a few interesting differences. The most striking differences were the loss of one histidine and one methionine in the isoenzyme, corresponding to residues 24 and 27, respectively, in alpha-phospholipase A2. The positional and stereo specificity of both enzymes are the same, but the specific activity of the beta-phospholipase A2 is lower. The molecular weight of the isoenzyme was estimated to be about 14 000, while the isoelectric points were 5.1 and 5.9 for the isoprecursor and active isoenzyme, respectively.

  • References11
  • Citations12

References

Mentioned in this Paper

PLA2G2A protein, human
Phospholipase A2, human
Histidine
Enzymes, antithrombotic
Chromatography
Benign Tumor of Pancreas
PLA2G4C protein, human
Prophospholipase A2
Pedameth
Enzymes for Treatment of Wounds and Ulcers

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