Phospholipase activation and arachidonic acid release in cultured intestinal epithelial cells (INT 407)

Scandinavian Journal of Gastroenterology
C Gustafson, C Tagesson

Abstract

The release of free arachidonic acid (AA) in cultured intestinal epithelial cells (INT 407) was investigated. INT-407 cells were first incubated overnight with radiolabeled 14C-AA, and most of the incorporated 14C-AA esterified into phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol. Labeled cells were then exposed to different stimulating agents and the release of free 14C-AA determined. The calcium ionophore A23187 caused a dose-dependent AA release that was preceded by a rapid uptake and a subsequent efflux of 45Ca2+. By contrast, phospholipase C from Clostridium perfringens caused a great AA release that was accompanied by an apparent uptake and a sustained intracellular accumulation of 45Ca2+. The cells alos released AA when exposed to the protein kinase C activator, 4 beta-phorbol-12-myristate-13-acetate (PMA), and this agent, like the diacylglycerol 1-oleoyl-2-acetyl-rac-glycerol, significantly potentiated the AA release caused by A23187. Not only A23187-mediated but also phospholipase C- and PMA-mediated AA release was inhibited by 4-bromophenacyl bromide, a known phospholipase A2 inhibitor. These findings, taken together, indicate that AA release in intestinal epithelial cells can be caused by (i) ...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L BellP W Majerus
Jan 1, 1979·Gut·T A Miller, E D Jacobson
Apr 1, 1986·Archives of Biochemistry and Biophysics·D M Smith, M Waite
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·A AlyC Johansson
May 1, 1988·Scandinavian Journal of Gastroenterology·C GustafsonC Tagesson
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·J Rask-Madsen
Jul 31, 1985·Biochemical and Biophysical Research Communications·A Mobley, H H Tai
Jan 6, 1973·British Medical Journal·G SigurdssonG F Joplin
Apr 30, 1971·Annals of the New York Academy of Sciences·H Kunze, W Vogt
Dec 1, 1984·Gut·D S Rampton, C J Hawkey
Dec 16, 1983·Biochemical and Biophysical Research Communications·W ZawalichH Rasmussen
Sep 30, 1980·Biochimica Et Biophysica Acta·H van den Bosch
Nov 30, 1983·Biochemical and Biophysical Research Communications·R M DawsonR F Irvine
Oct 31, 1983·Biochemical and Biophysical Research Communications·I KojimaH Rasmussen
Sep 30, 1980·Biochemical and Biophysical Research Communications·R KannagiT Masuda

❮ Previous
Next ❯

Citations

Feb 1, 1997·The Journal of Laboratory and Clinical Medicine·W LieberthalJ H Schwartz
Jan 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·G Nygård, T Berglindh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Trends in Pharmacological Sciences
K Kohama
Journal of Peptide Science : an Official Publication of the European Peptide Society
Mark G DevlinDavid P Fairlie
© 2022 Meta ULC. All rights reserved