Phospholipase C activity affinity purifies with the Torpedo nicotinic acetylcholine receptor.

The Journal of Biological Chemistry
Jonathan M LabriolaJohn E Baenziger

Abstract

Nicotinic acetylcholine receptors mediate fast synaptic transmission by fluxing ions across the membrane in response to neurotransmitter binding. We show here that during affinity purification of the nicotinic acetylcholine receptor from Torpedo, phosphatidic acid, but not other anionic or zwitterionic phospholipids, is hydrolyzed to diacylglycerol. The phospholipase C activity elutes with the acetylcholine receptor and is inhibited by a lipid phosphate phosphohydrolase inhibitor, sodium vanadate, but not a phosphatidate phosphohydrolase inhibitor, N-ethylmaleimide. Further, the hydrolysis product of phosphatidic acid, diacylglycerol, enhances the functional capabilities of the acetylcholine receptor in the presence of anionic lipids. We conclude that a phospholipase C activity, which appears to be specific for phosphatidic acid, is associated with the nicotinic acetylcholine receptor. The acetylcholine receptor may directly or indirectly influence lipid metabolism in a manner that enhances its own function.

References

Sep 15, 1976·The Biochemical Journal·J E BleasdaleE Heilbronn
Sep 12, 1998·The Journal of Biological Chemistry·D N Brindley, D W Waggoner
Feb 12, 2002·Nature Reviews. Neuroscience·Arthur Karlin
Nov 19, 2002·Journal of Neurobiology·Geeta Sharma, Sukumar Vijayaraghavan
Dec 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Corrie J B daCosta, John E Baenziger
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Mar 24, 2006·Nature·Steven M Sine, Andrew G Engel
May 11, 2006·Annals of Neurology·Xin-Ming ShenAndrew G Engel
Jun 20, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marie-Renée BlanchetYvon Cormier
Nov 1, 2006·Current Pharmaceutical Design·A J Thompson, S C R Lummis
May 15, 2007·Progress in Brain Research·T GoetzP Wulff
May 16, 2007·British Journal of Pharmacology·W J de Jonge, L Ulloa
May 2, 2008·Chemical Reviews·Dennis A Dougherty
Apr 10, 2009·The Journal of Biological Chemistry·Corrie J B daCosta, John E Baenziger
Oct 10, 2009·The Journal of Biological Chemistry·Corrie J B daCostaJohn E Baenziger
Mar 23, 2010·Biophysical Journal·Raymond M Sturgeon, John E Baenziger

❮ Previous
Next ❯

Citations

Sep 10, 2013·Nature Chemical Biology·Corrie J B daCostaJohn E Baenziger
Mar 23, 2010·Biophysical Journal·Raymond M Sturgeon, John E Baenziger
Mar 21, 2015·Biochimica Et Biophysica Acta·John E BaenzigerJiayin Sun
Sep 18, 2018·Pakistan Journal of Biological Sciences : PJBS·Wuyi Liu, Kunkai Su
Apr 21, 2020·Biochimica Et Biophysica Acta. Biomembranes·Mackenzie J Thompson, John E Baenziger

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.