Phospholipase C delta-type consists of three isozymes: bovine PLCdelta2 is a homologue of human/mouse PLCdelta4

Biochemical and Biophysical Research Communications
Yasuhiro IrinoK Fukami

Abstract

To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, beta-, gamma-, delta-, epsilon-, and zeta-type. PLCdelta-type is reported to be composed of four isozymes, PLCdelta1-delta4. Here we report that a screening for mouse PLCdelta2 from a BAC library with primers that amplify a specific region of bovine PLCdelta2 resulted in isolation of one clone containing the mouse PLCdelta4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCdelta2 and PLCdelta4 in the mouse and human genomes, indicating that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4. However, PLCdelta2 Western blot analysis with a widely used commercial anti-PLCdelta2 antibody showed an expression pattern distinct from that of PLCdelta4 in wild-type mice. In addition, an 80-kDa band, which was recognized by antibody against PLCdelta2, was smaller than an 85-kDa band detected by anti-PLCdelta4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCdelta4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCdelta2 antibody contained no PLC activity. From these data, we conclude that bo...Continue Reading

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Citations

Jun 6, 2008·Molecular Neurobiology·Tibor RohacsViktor Lukacs
Aug 21, 2013·Advances in Biological Regulation·Yoshikazu NakamuraKiyoko Fukami
Dec 29, 2010·The Journal of Biological Chemistry·Zen KouchiKiyoko Fukami
Nov 30, 2005·Molecular and Cellular Biology·Yoshikazu NakamuraKiyoko Fukami
Jun 24, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Christopher J ClarkeJacqueline Ohanian
May 1, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Na Young KimJoon Ki Chung
Sep 14, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hye Jin BakJoon Ki Chung
Nov 16, 2012·Neuroscience Bulletin·Vincenza Rita Lo Vasco
Jan 12, 2010·Gene Expression Patterns : GEP·Kaori KanemaruKiyoko Fukami
Dec 8, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Moo Sang KimHyung Ho Lee
Jul 3, 2008·BMB Reports·Pann-Ghill SuhSung Ho Ryu
Aug 30, 2008·Genomics·Moo-Sang KimJoon Ki Chung
Jul 31, 2013·Gene·Na Young KimJoon Ki Chung
Apr 3, 2007·Biochemical and Biophysical Research Communications·Manabu IchinoheKiyoko Fukami
Oct 26, 2006·Journal of Cellular Biochemistry·Vincenza Rita Lo VascoFrancesco Antonio Manzoli
Aug 15, 2009·Journal of Cellular Biochemistry·Makoto FujiiHitoshi Yagisawa
Nov 8, 2005·Journal of Biochemistry·Yasuhiro IrinoKiyoko Fukami
Aug 1, 2013·Physiological Reviews·Tamas Balla
Sep 24, 2020·Progress in Lipid Research·Matilda Katan, Shamshad Cockcroft
Nov 29, 2005·Trends in Biochemical Sciences·Shamshad Cockcroft
Jan 8, 2008·Advances in Enzyme Regulation·Kiyoko FukamiYoshikazu Nakamura

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