Phospholipase C-gamma1 is required for cell survival in oxidative stress by protein kinase C

The Biochemical Journal
Xiao-chun BaiShen-qiu Luo

Abstract

Phospholipase C-gamma1 (PLC-gamma1) activation has been reported to enhance cell survival during the cellular response to oxidative stress. We studied the role of protein kinase C (PKC) pathways in mediating PLC-gamma1 survival signalling in oxidative stress by using mouse embryonic fibroblasts genetically deficient in PLC-gamma1 (Plcg1(-/-)) and its wild type (Plcg1(+/+)). PLC-gamma1 was activated by H(2)O(2) treatment in a dose- and time-dependent manner. Activation of PKC was also markedly increased in both cell lines treated with H(2)O(2) (1-5 mM), but with low doses (50-200 microM), PKC activation was considerably decreased in Plcg1(-/-) cells. After treatment with H(2)O(2), PKC-dependent phosphorylation of Bcl-2 and cell viability of Plcg1(-/-) cells decreased dramatically and caspase-3-like activity increased significantly compared with that of the wild-type cells. Furthermore, pretreatment of Plcg1(+/+) cells with PKC-specific inhibitor decreased levels of PKC-dependent Bcl-2 phosphorylation, enhanced caspase-3 activity and their sensitivity to H(2)O(2). On the contrary, treatment of Plcg1(-/-) cells with PKC-specific activator increased the Bcl-2 phosphorylation, decreased caspase-3 activity and improved their survival...Continue Reading

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Citations

Jan 13, 2004·Biochemical and Biophysical Research Communications·Xiao-chun BaiShen-qiu Luo
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carlos E IrarrazabalJoan D Ferraris
Nov 16, 2007·Kidney & Blood Pressure Research·Zhangping Peng, William J Arendshorst
May 3, 2008·Biological & Pharmaceutical Bulletin·Kentaro NishidaSadaki Fujimoto
Aug 4, 2005·BMC Biology·Carrie Dickey-SimsJames A Coffman
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Jul 3, 2008·BMB Reports·Pann-Ghill SuhSung Ho Ryu
Feb 26, 2005·The Journal of Biological Chemistry·Xiao-chun BaiShen-qiu Luo
Aug 25, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Gloria Gutiérrez-VenegasJosé Antonio Méndez-Mejía
Oct 4, 2011·Advances in Biological Regulation·Yong Ryoul YangPann-Ghill Suh
Oct 12, 2011·Biochemical and Biophysical Research Communications·Hee Jun ChoJiyun Yoo

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