PMID: 9182729Jun 1, 1997Paper

Phospholipase C inhibitor, U73122, releases intracellular Ca2+, potentiates Ins(1,4,5)P3-mediated Ca2+ release and directly activates ion channels in mouse pancreatic acinar cells

The Biochemical Journal
H MogamiD V Gallacher

Abstract

It is recognized in many cellular systems that the receptor/G-protein activation of phospholipase C and Ins(1,4,5)P3 production is the transduction pathway regulating the release of Ca2+ from internal stores. Ca2+ signals can now be monitored at the level of single cells but the biochemical detection of Ins(1,4,5)P3 cannot match this resolution. It is often difficult or impossible to directly attribute responses evoked in single cells by putative phospholipase C-coupled agonists to changes in Ins(1,4,5)P3 levels. U73122 is an aminosteroid that is reported to act as a specific inhibitor of phospholipase C and it has become an important tool in establishing the link between phospholipase C activation and cellular Ca2+ signalling. In the present study we use both patch-clamp electrophysiology and the imaging of fluorescent Ca2+ indicators to investigate the effect of U73122 in mouse pancreatic acinar cells. The study reveals that U73122 has effects other than the inhibition of phospholipase C. U73122 can directly activate ion channels. It can itself promote the release of Ca2+ from intracellular stores in permeabilized cells and in intact cells it triggers a release of Ca2+ that is initiated specifically at the secretory pole of t...Continue Reading

Citations

Sep 1, 2005·The Journal of General Physiology·Lisa F HorowitzBertil Hille
Nov 5, 2014·Journal of Pharmacological Sciences·Masayuki X Mori, Ryuji Inoue
Feb 16, 2017·PLoS Computational Biology·Jung Min HanJames Sneyd
Oct 9, 2007·British Journal of Pharmacology·B T McIntoshM E M Kelly
Mar 27, 2003·Alcoholism, Clinical and Experimental Research·Katherine A Debelak-KragtorpSusan M Smith
Apr 7, 2017·American Journal of Physiology. Cell Physiology·Allen J YiuBidhan C Bandyopadhyay
Feb 23, 2012·The EMBO Journal·Christoph BrenkerTimo Strünker
Nov 20, 2015·Molecular Nutrition & Food Research·Manuela MeirelesConceição Calhau
Sep 4, 2015·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Chang Ho MinUy Dong Sohn
Nov 4, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·E A MacRobbie
Jan 23, 2019·CNS Neuroscience & Therapeutics·Francisco M Torres-CruzElizabeth Hernández-Echeagaray
Jan 19, 2010·Molecular Plant Pathology·Zafer Dallal BashiMohammed H Borhan
Jun 3, 2005·The Journal of Biological Chemistry·Sougata KarmakarRodger P McEver
Feb 20, 2009·Molecular and Cellular Biochemistry·Tushi SingalParamjit S Tappia
Jul 31, 2013·Zygote : the Biology of Gametes and Early Embryos·María de Los Angeles SerranoSara Serafina Sánchez
Oct 21, 2009·The Journal of Physiology·Hitoshi IshibashiJunichi Nabekura
Jun 4, 2014·Alcoholism, Clinical and Experimental Research·Jay M BolnickD Randall Armant
Dec 15, 2015·Frontiers in Cardiovascular Medicine·Danielle KamatoPeter J Little
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·E A MacRobbie
Jan 11, 2016·Molecular Microbiology·Mathieu Brochet, Oliver Billker
Mar 5, 2020·Biomolecules & Therapeutics·Wynn TheinUy Dong Sohn
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·N J BramichG D Hirst
Mar 27, 2019·Journal of Cell Science·Cliff-Lawrence IbehBidhan C Bandyopadhyay
May 22, 2003·American Journal of Physiology. Endocrinology and Metabolism·Cecilia SuárezDamasia Becu-Villalobos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.