PMID: 9422360Jan 9, 1998Paper

Phospholipase Cgamma1 in bovine rod outer segments: immunolocalization and light-dependent binding to membranes

Journal of Neurochemistry
Abboud J GhalayiniRobert E Anderson

Abstract

We have investigated the isozymes of a phosphoinositide-specific phospholipase C (PLC) in bovine retina using several monoclonal antisera to PLCbeta1, gamma1, and delta1. Immunoblot analysis showed that all three isozymes were present in the retina. Immunocytochemical localization in frozen bovine retina sections showed that PLCgamma1 was present in the photoreceptor cell layer, outer plexiform cell layer, inner plexiform cell layer, and ganglion cell layer. Immunoreaction within the photoreceptor cell layer was dependent on dark/light adaptation state of retinas. Immunoblot analysis of rod outer segments (ROS) with monoclonal or polyclonal antibodies to PLCgamma1 showed the presence of an immunoreactive band of 140 kDa. ROS prepared from retinas light-adapted in vitro had more PLCgamma1 on immunoblots than ROS from dark-adapted retinas. PLC enzyme activity in ROS from light-adapted retinas was 69 and 46% higher than ROS from dark-adapted retinas, when assayed in the presence and absence of ATP, respectively. This increase in enzyme activity was observed at [Ca2+]free between 0.32 and 100 microM. These results demonstrate the presence of PLCgamma1 in bovine ROS and show that ROS prepared from light-adapted retinas are enriched ...Continue Reading

Citations

Nov 14, 2001·The Journal of Biological Chemistry·Abboud J GhalayiniHisaaki Kawakatsu
Oct 16, 2009·Journal of Lipid Research·Norma M GiustoMónica G Ilincheta de Boschero
Sep 20, 2011·Molecular Neurobiology·Susan E Brockerhoff
Jun 11, 2020·Cells·Stella FinkelsteinEkaterina S Lobanova
Jun 15, 2000·Journal of Neurochemistry·Z HuangR E Anderson
Dec 31, 2003·The Journal of Biological Chemistry·Feng HeTheodore G Wensel
Jun 17, 2020·Journal of Lipid Research·Raju V S Rajala
Feb 9, 2021·Journal of Lipid Research·Raju V S Rajala
Nov 10, 2015·Experimental Eye Research·Paola M NataliniNorma M Giusto
Jul 19, 2000·Archives of Biochemistry and Biophysics·S J PasquaréN M Giusto
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Jun 14, 2002·Archives of Biochemistry and Biophysics·P I Castagnet, N M Giusto

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