Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana

Frontiers in Plant Science
Martin JandaOlga Valentová

Abstract

Phytohormone salicylic acid (SA) is a crucial component of plant-induced defense against biotrophic pathogens. Although the key players of the SA pathway are known, there are still gaps in the understanding of the molecular mechanism and the regulation of particular steps. In our previous research, we showed in Arabidopsis suspension cells that n-butanol, which specifically modulates phospholipase D activity, significantly suppresses the transcription of the pathogenesis related (PR-1) gene, which is generally accepted as the SA pathway marker. In the presented study, we have investigated the site of n-butanol action in the SA pathway. We were able to show in Arabidopsis plants treated with SA that n-butanol inhibits the transcription of defense genes (PR-1, WRKY38). Fluorescence microscopy of Arabidopsis thaliana mutants expressing 35S::NPR1-GFP (nonexpressor pathogenesis related 1) revealed significantly decreased nuclear localization of NPR1 in the presence of n-butanol. On the other hand, n-butanol did not decrease the nuclear localization of NPR1 in 35S::npr1C82A-GFP and 35S::npr1C216A-GFP mutants constitutively expressing NPR1 monomers. Mass spectrometric analysis of plant extracts showed that n-butanol significantly chan...Continue Reading

References

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Citations

Apr 27, 2019·Frontiers in Plant Science·Tomáš TakáčJozef Šamaj
Sep 7, 2019·International Journal of Molecular Sciences·Igor PokotyloEric Ruelland
Jul 30, 2020·International Journal of Molecular Sciences·Beatriz A Rodas-JuncoS M Teresa Hernández-Sotomayor
Dec 15, 2020·Trends in Plant Science·Jingjing XingJinxing Lin

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Methods Mentioned

BETA
transgenic
PCR
PCA
confocal microscopy

Software Mentioned

Genevestigator
MarkerLynx
APS Asess
APS Assess
LightCycler
PerlPrimer
MassLynx
STATGRAPHICS® Centurion XVII

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