Phospholipase D family interactions with the cytoskeleton: isoform delta promotes plasma membrane anchoring of cortical microtubules

Functional Plant Biology : FPB
Z AndreevaJan Marc

Abstract

Phospholipase D (PLD) is a key enzyme in signal transduction - mediating plant responses to various environmental stresses including drought and salinity. Isotype PLDδ interacts with the microtubule cytoskeleton, although it is unclear if, or how, each of the 12 PLD isotypes in Arabidopsis may be involved mechanistically. We employed RNA interference in epidermal cells of Allium porrum L. (leek) leaves, in which the developmental reorientation of cortical microtubule arrays to a longitudinal direction is highly sensitive to experimental manipulation. Using particle bombardment and transient transformation with synthetic siRNAs targeting AtPLDα, β, γ, δ, ॉ and ζ, we examined the effect of 'cross-target' silencing orthologous A. porrum genes on microtubule reorientation dynamics during cell elongation. Co-transformation of individual siRNAs together with a GFP-MBD microtubule-reporter gene revealed that siRNAs targeting AtPLDδ promoted, whereas siRNAs targeting AtPLDβ and γ reduced, longitudinal microtubule orientation in A. porrum. These PLD isotypes, therefore, interact, directly or indirectly, with the cytoskeleton and the microtubule-plasma membrane interface. The unique response of PLDδ to silencing, along with its exclusive...Continue Reading

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Citations

Aug 31, 2012·Planta·Ayelen M DistéfanoAna M Laxalt
Feb 18, 2020·The Plant Journal : for Cell and Molecular Biology·Přemysl PejcharMartin Potocký
Nov 25, 2017·Journal of Plant Research·Yoshihisa Oda
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Dec 15, 2020·Plant, Cell & Environment·Scott HayesSteven A Arisz
Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang

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