Phospholipase D from photoreceptor rod outer segments is a downstream effector of RhoA: evidence of a light-dependent mechanism

Experimental Eye Research
G A Salvador, N M Giusto

Abstract

Photoreceptor cells contain rod outer segments (ROS) which are specialized light-sensitive organelles. The biological function of ROS is to generate a photoresponse, which occurs via the classic transducin-mediated pathway. Moreover, ROS undergo light-regulated membrane turnover and protein translocation whose mechanisms have not been fully elucidated to date. Phospholipase D (PLD) is a key enzyme involved in lipid signal transduction and membrane trafficking. We have previously reported that PLD activity is present in purified ROS (Salvador, G.A., Giusto, N.M., 1998. Characterization of phospholipase D activity in bovine photoreceptor membranes. Lipids 33, 853-860). We now demonstrate that ROS PLD activity is enhanced by phosphatidylinositol bisphosphate (PIP2) and cytosolic factors in a GTP dependent-manner. Western blot analysis demonstrates the presence of PLD1 isoform in purified ROS. In ROS obtained from dark-adapted retinas (DROS), PIP2-dependent PLD activity was higher than that observed in ROS obtained from light-adapted retinas (LROS). In addition, experiments carried out in the presence of C3 toxin inhibited PLD activity from DROS whereas pertussis toxin did not affect the enzyme activity. Western blot analysis demon...Continue Reading

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Citations

Feb 22, 2012·Biochemistry. Biokhimii︠a︡·Y S KolesnikovV S Kravets
Oct 16, 2009·Journal of Lipid Research·Norma M GiustoMónica G Ilincheta de Boschero
Apr 14, 2012·Trends in Neurosciences·Craig Montell
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Apr 25, 2019·Molecular Neurobiology·Estefanía Chamorro AguirreSusana J Pasquaré
Jun 17, 2020·Journal of Lipid Research·Raju V S Rajala
Feb 9, 2021·Journal of Lipid Research·Raju V S Rajala

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