Phospholipase D in human platelets: presence of isoenzymes and participation of autocrine stimulation during thrombin activation

Platelets
M Vorland, Holm Holmsen

Abstract

Phospholipase D (PLD), which hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline, is present in human platelets. Thrombin and other agonists have been shown to activate PLD but the precise mechanisms of activation and PLDs role in platelet activation remains unclear. We measured thrombin-stimulated PLD activity in platelets as formation of phosphatidylethanol. Since no specific PLD inhibitors exist, we investigated possible roles for PLD in platelets by correlating PLD activity with platelet responses such as thrombin-mediated secretion and F-actin formation (part of platelet shape change). Extracellular Ca2+ potentiated thrombin-stimulated PLD, but did not stimulate PLD in the absence of thrombin. Thrombin-induced PLD activity was enhanced by secreted ADP and binding of fibrinogen to its receptors. In contrast to others, we also found a basal PLD activity. Comparison of time courses and dose responses of platelets with PLD showed many points of correlation between PLD activation and lysosomal secretion and F-actin formation. The finding of different PLD activities suggested that different PLD isoenzymes exist in platelets as reported for other cells. Here we present evidence for the presence of both PLD1 and P...Continue Reading

References

Jan 1, 1989·Methods in Enzymology·H Holmsen, C A Dangelmaier
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A AhmedB J Holub
Nov 15, 1988·Biochemical and Biophysical Research Communications·R Rubin
Nov 1, 1988·Thrombosis Research·N FukamachiB Kobayashi
May 15, 1995·FEBS Letters·Y M Heung, A D Postle
Feb 6, 1995·Biochemical and Biophysical Research Communications·J R NoferG Assmann
Jan 1, 1993·Advances in Experimental Medicine and Biology·R J Haslam, J R Coorssen
Jan 17, 1996·International Journal of Cancer. Journal International Du Cancer·G O NessJ R Lillehaug
May 24, 1996·Chemistry and Physics of Lipids·S P HalendaS D Shukla
Sep 1, 1996·Journal of Lipid Mediators and Cell Signalling·D LangL Freysz
Apr 25, 1997·The Journal of Biological Chemistry·T Kodaki, S Yamashita
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M SteedD J Lasala
Nov 25, 1998·Biochemical and Biophysical Research Communications·Z Qingqi, A Stracher
Dec 29, 1998·The Journal of Biological Chemistry·T C SungM A Frohman
Feb 26, 1999·The Journal of Biological Chemistry·M YamazakiY Kanaho
Jul 30, 1999·Biochimica Et Biophysica Acta·M LiscovitchX Tang
Apr 5, 2000·The Journal of Biological Chemistry·S J WalkerH A Brown
Mar 22, 2001·The Journal of Biological Chemistry·K OishiY Ono
Mar 29, 2001·Traffic·D A SchaferJ A Cooper
May 25, 2001·The Journal of Biological Chemistry·S LeeS H Ryu
Nov 15, 2001·Cellular and Molecular Life Sciences : CMLS·S Cockcroft

❮ Previous
Next ❯

Citations

Mar 31, 2011·Acta Physiologica·X Peng, M A Frohman
Nov 18, 2008·Platelets·M VorlandH Holmsen
May 15, 2012·Cellular Signalling·Margitta ElversKnut Fälker
Sep 15, 2012·Journal of Thrombosis and Haemostasis : JTH·I ThielmannB Nieswandt
Jun 15, 2016·PloS One·Jonathan Trujillo VieraGrzegorz Sumara
Jul 23, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·David StegnerBernhard Nieswandt
Nov 9, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·David Stegner, Bernhard Nieswandt
Sep 18, 2013·The Journal of Pharmacology and Experimental Therapeutics·Elke C G JacksonArchie McNicol
Apr 15, 2014·Digestive Diseases and Sciences·Xinyan ZhuWenzhuo Yang
Nov 18, 2018·Journal of Clinical Medicine·Wan Jung LuJoen Rong Sheu
Jul 6, 2014·The Journal of Biological Chemistry·Yi Zhang, Michael A Frohman
Dec 13, 2019·Progress in Lipid Research·M I McDermottV A Bankaitis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.