Phospholipase D δ knock-out mutants are tolerant to severe drought stress

Plant Signaling & Behavior
Ayelen M DistéfanoAna M Laxalt

Abstract

Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stress to plant development. Phospholipase Dδ (PLDδ) is activated in dehydration and salt stress, producing the lipid second messenger phosphatidic acid. In this work we show that pldδ Arabidopsis mutants were more tolerant to severe drought than wild-type plants. PLDδ has been shown to be required for ABA regulation of stomatal closure of isolated epidermal peels. However, there was no significant difference in stomatal conductance at the whole plant level between wild-type and pldδ mutants. Since PLD hydrolyses structural phospholipids, then we looked at membrane integrity. Ion leakage measurements showed that during dehydration of leaf discs pldδ mutant has less membrane degradation compared to the wild-type. We further analyzed the mutants and showed that pldδ have higher mRNA levels of RAB18 and RD29A compared to wild-type plants under normal growth conditions. Transient expression of AtPLDδ in Nicotiana benthamiana plants induced a wilting phenotype. These findings suggest that, in wt plants PLDδ disrupt membranes in severe drought stress and, in the absence of the protein (PLDδ knock-out) might drought-prime the p...Continue Reading

References

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Citations

Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang
Nov 23, 2016·Current Opinion in Plant Biology·Dave StatecznyPeter Bommert
May 8, 2016·Biochimica Et Biophysica Acta·Amélie A Kelly, Ivo Feussner
Nov 18, 2017·Journal of Experimental Botany·Mariana RegenteLaura de la Canal
Sep 14, 2017·Journal of Experimental Botany·Eun Hyang HanJoshua J Blakeslee
Mar 4, 2021·International Journal of Molecular Sciences·Evangelia StavridouIrini Nianiou-Obeidat
Apr 4, 2021·International Journal of Molecular Sciences·Beatriz A Rodas-JuncoS M Teresa Hernández-Sotomayor
Nov 15, 2020·Biochemical and Biophysical Research Communications·Qin LiuShuyang Wang

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Datasets Mentioned

BETA
U14432

Methods Mentioned

BETA
PCR
transgenic
electrophoresis

Software Mentioned

Stepone

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