Phospholipase D regulation by a physical interaction with the actin-binding protein gelsolin

Biochemistry
P M SteedL P Wennogle

Abstract

Increases in intracellular phosphatidic acid levels caused by receptor- mediated activation of phospholipase D (PLD) have been implicated in many signal transduction pathways leading to cellular activation. PLD is known to be regulated by several means, including tyrosine kinase activity, increases in Ca2+, receptor-coupled G proteins, small GTP binding proteins, ceramide metabolisms, and protein kinase C. We have investigated a additional regulatory effect on PLD activity involving nucleoside triphosphates (NTPs). A NTP binding protein copurifies with LPD activity from rabbit brains using a GTP-agarose affinity column, and this protein stimulates PLD activity only in the absence of NPTs. The NTP effect is reversible and labile, and the binding protein is separable from the PLD activity by heparin-agarose chromatography. We identified this protein as the actin- binding protein gelsolin by amino acid sequencing following peptide mapping. This finding was verified by the co-immunoprecipitation of gelsolin and PLD activity as well as by the reconstitution of gelsolin- dependent nucleotide sensitive PLD activity by the addition of purified gelsolin-free PLD. Our data indicate that actin rearrangements and PLD signaling are coordina...Continue Reading

References

Feb 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S L ReinholdT M McIntyre
Oct 1, 1987·The Journal of Cell Biology·J A Cooper
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Feb 1, 1994·Current Opinion in Cell Biology·M D RozyckiU Lindberg
Jan 17, 1994·FEBS Letters·J N Kanfer, D McCartney
Aug 1, 1993·The Biochemical Journal·J Stutchfield, S Cockcroft
Oct 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J Gomez-Cambronero
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Aug 4, 1999·Molecular and Cellular Endocrinology·A M StrandH S Hansen
Feb 27, 1999·Current Opinion in Cell Biology·D J Kwiatkowski
Oct 2, 2003·European Journal of Biochemistry·Emeline LagarrigueClaude Roustan
Oct 13, 2010·The Journal of Biological Chemistry·Maya FinkelsteinHaim Breitbart
Apr 16, 2002·Molecular Biology of the Cell·Hélène DefacqueGareth Griffiths
Dec 18, 2002·The EMBO Journal·Veerle De CorteJan Gettemans
Apr 21, 1999·Protein Science : a Publication of the Protein Society·V De CorteJ Vandekerckhove
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·Y S KolesnikovV S Kravets
May 13, 2010·Biological & Pharmaceutical Bulletin·Atsuko KawajiMasayoshi Imagawa
Jan 7, 2000·Biochemical and Biophysical Research Communications·J Y Kwak, D J Uhlinger
Aug 25, 1997·The Journal of Cell Biology·H q SunH L Yin
May 10, 2000·FEBS Letters·S K MaciverP J McLaughlin
Mar 21, 2009·Biochimica Et Biophysica Acta·Shamshad Cockcroft
Aug 14, 2012·Medicinal Research Reviews·Guo Hua LiPeter Liu
May 25, 2002·Experimental Cell Research·Yan DongBonnie B Asch
May 18, 1999·Experimental Cell Research·L M MielnickiB B Asch
Nov 25, 1998·Biophysical Journal·H J KinosianJ E Estes
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mark McDermottAndrew J Morris
Aug 15, 1997·The Journal of Biological Chemistry·K M LinH L Yin
Nov 19, 2004·The Journal of Biological Chemistry·Young Chan ChaeSung Ho Ryu
Jan 21, 2010·Physiological Reviews·Juha SaarikangasPekka Lappalainen
Dec 14, 2001·The Journal of Biological Chemistry·Sukmook LeeSung Ho Ryu
Jun 20, 1997·The Journal of Biological Chemistry·J H Exton
Aug 15, 2001·American Journal of Physiology. Cell Physiology·A PanebraS Khurana
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M SteedD J Lasala
May 25, 2001·The Journal of Biological Chemistry·S LeeS H Ryu
Oct 28, 1998·Physiological Reviews·E J Pettit, F S Fay
Jan 27, 2021·Advances in Biological Regulation·Forrest Z BowlingMichael V Airola

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