Phospholipase D1 regulates phagocyte adhesion

The Journal of Immunology : Official Journal of the American Association of Immunologists
Shankar S IyerD J Kusner

Abstract

Adhesion is a fundamental cellular response that is essential to the physiologic processes of development, differentiation, proliferation, and motility, as well as to the pathology of inflammation, transformation, and metastasis. Adhesion of phagocytic leukocytes is a critical modulator of antimicrobial and cytotoxic functions, including the respiratory burst, secretion, and apoptosis. Because phospholipase D (PLD) is linked to several signaling pathways implicated in these processes, we tested the hypothesis that PLD regulates phagocyte adhesion. Adhesion of primary human neutrophils and monocyte-derived macrophages to fibronectin was accompanied by marked stimulation of PLD activity. Similarly, adhesion of both human (PLB, THP-1) and murine (RAW) myeloid-macrophage cell lines to fibronectin, fibrinogen, collagen, or plastic resulted in significant activation of PLD. Stimulation of PLD activity was rapid and persisted for at least 90 min. Confocal microscopy indicated that PLD1 exhibited partial colocalization with actin filaments at the adherent interface, in proximity to the focal adhesion protein, paxillin. Reductions in PLD activity by chemical inhibitors or specific short-interfering RNA-induced knockdown of PLD1 resulted...Continue Reading

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Citations

Jan 25, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Paschal A Oude WeerninkMartina Schmidt
Nov 18, 2008·Platelets·M VorlandH Holmsen
Sep 5, 2014·The Journal of Biological Chemistry·Francis SperanzaJulian Gomez-Cambronero
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Oct 24, 2006·Biochimica Et Biophysica Acta·Paschal A Oude WeerninkMartina Schmidt
Mar 20, 2009·Veterinary Immunology and Immunopathology·Saori KobayashiJuso Sasaki

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