Phospholipase D2 stimulates cell protrusion in v-Src-transformed cells

Biochemical and Biophysical Research Communications
Yingjie ShenDavid A Foster


Phospholipase D (PLD) activity has been implicated in several aspects of cell physiology including vesicle transport, signal transduction, cell proliferation, cytoskeletal structure, and oncogenic transformation. Two PLD isoforms (PLD1 and PLD2) have been identified and characterized. We have expressed both wild-type and catalytically inactive forms of PLD1 and PLD2 in 3Y1 rat fibroblasts and in 3Y1 cells transformed by v-Src, a tyrosine kinase that elevates PLD activity. The v-Src-transformed 3Y1 cells have small, but distinct cell protrusions, implicated in cell migration and metastasis. We report here that elevated expression of PLD2 substantially increased the length of the cell protrusions and that a catalytically inactive PLD2 mutant abolished the cell protrusions. The extended protrusions in the PLD2-overexpressing cells were dependent upon microtubule assembly. These data suggest a role for PLD2 in the v-Src-mediated formation of cell protrusions that may be critical for the invasive properties of v-Src-transformed cells.


Mar 1, 1988·Clinical & Experimental Metastasis·E PrangeH Pralle
Jan 1, 1984·International Review of Cytology·M M Mareel, M De Mets
Jan 1, 1993·Journal of Molecular Neuroscience : MN·M E Cox, P F Maness
Mar 1, 1995·Clinical & Experimental Metastasis·R ReichM Liscovitch
Jun 15, 1993·Biochemical and Biophysical Research Communications·F ImamuraH Akedo
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
May 16, 1997·The Journal of Biological Chemistry·Q ZangD A Foster
May 29, 1997·International Journal of Cancer. Journal International Du Cancer·J A Aguirre GhisoE Bal de Kier Joffé
Jun 27, 1997·Biochemical and Biophysical Research Communications·J Q LuoD A Foster
Nov 5, 1997·Analytical Biochemistry·A J MorrisJ Engebrecht
May 14, 1998·International Journal of Cancer. Journal International Du Cancer·J A Aguirre GhisoE Bal de Kier Joffé
Jul 31, 1998·Experimental Cell Research·V LadedaE Bal de Kier Joffé
Feb 27, 1999·Current Opinion in Cell Biology·C M Waterman-Storer, E Salmon
Jun 8, 1999·Chemistry and Physics of Lipids·S Nakashima, Y Nozawa
Jun 22, 1999·FEBS Letters·J V SmallK Rottner
Dec 28, 1999·Science·A R Horwitz, J T Parsons
Feb 19, 2000·Current Opinion in Cell Biology·B L GoodeG Barnes
Nov 25, 2000·Cancer Letters·D Y NohJ S Han
Dec 15, 2000·Oncogene·J D BjorgeD J Fujita
Dec 15, 2000·Oncogene·R B Irby, T J Yeatman
Jan 3, 2001·Molecular and Cellular Biology·Y ShenD A Foster
Jan 13, 2001·Genes & Development·E Fuchs, I Karakesisoglou
Feb 24, 2001·Biochemical and Biophysical Research Communications·Y ZhaoY Nozawa
Mar 29, 2001·Traffic·D A SchaferJ A Cooper
Apr 27, 2001·Seminars in Cancer Biology·J KassisA Wells
May 15, 2001·Biochemical and Biophysical Research Communications·V LadedaJ A Aguirre-Ghiso
May 22, 2001·Molecular and Cellular Biology·Y Kam, J H Exton

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