Abstract
D type phospholipases (PLD) are enzymes that hydrolyze the head group of phospholipids to produce phosphatidic acid. This activity is ubiquitous in plant tissues, and has been isolated and characterized from different species and organs. Several families of these proteins have been described in plants on the basis of their gene sequences (PLD alpha, beta, gamma, delta, zeta and epsilon). They have been shown to be involved in many metabolic events, such as response to abiotic stress, signal transduction, and membrane lipid turnover and degradation. In the present study, PLD activity was measured in the soluble fractions isolated from different organs of this plant. A PLD of alpha type was cloned from leaf cDNA that was responsible for most of this activity. The gene encoding this 810 aa protein was heterologously expressed in E. coli. This protein was not lethal for the eukaryotic host, although it altered its phospholipid profile. PLDalpha was purified to almost homogeneity by His-tag affinity chromatography, displaying an optimum pH of 6.5 and strong dependence on the presence of Ca(2+) and SDS in the assay medium. The enzyme was active towards phosphatidylcholine, Phosphatidylethanolamine and phosphatidylglycerol. Furthermor...Continue Reading
References
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Nov 1, 1993·Archives of Biochemistry and Biophysics·X WangL Zheng
Nov 5, 1997·European Journal of Biochemistry·A AbousalhamR Verger
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Jan 23, 1998·The Plant Cell·L FanX Wang
May 20, 1998·The Plant Cell·V S Eccleston, J B Ohlrogge
Apr 2, 1999·Biochimica Et Biophysica Acta·D U KimM U Choi
Apr 10, 1999·Plant Physiology·L FanX Wang
Jul 30, 1999·Biochimica Et Biophysica Acta·K Pappan, X Wang
Aug 12, 1999·Archives of Biochemistry and Biophysics·K Pappan, X Wang
Apr 25, 2000·Progress in Lipid Research·X Wang
Apr 25, 2001·Physiologia Plantarum·Bruce D. WhitakerKaren C. Green
Nov 28, 2001·The Plant Journal : for Cell and Molecular Biology·Y SangX Wang
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 22, 2002·The Journal of Biological Chemistry·Ruth WeltiXuemin Wang
Aug 17, 2002·Current Opinion in Plant Biology·Xuemin Wang
May 1, 1996·Plant Physiology·L. XuX. Wang
Jun 1, 1994·Plant Physiology·J. H. DyerX. Wang
Jun 1, 1996·The Plant Cell·S. A. YoungJ. E. Leach
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Sep 26, 2003·The Plant Cell·Wenhua ZhangXuemin Wang
Nov 8, 2003·Progress in Nucleic Acid Research and Molecular Biology·Jean E Vance
Mar 9, 2004·Nature Biotechnology·Weiqi LiXuemin Wang
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wenhua ZhangXuemin Wang
Oct 13, 2005·Plant Physiology·Xuemin Wang
Nov 11, 2005·The FEBS Journal·Isaura SimõesEuclides Pires
Jul 18, 2006·Phytochemistry·Shivakumar Pattada DevaiahXuemin Wang
Sep 5, 2006·Journal of Plant Physiology·C B RajashekarXuemin Wang
Aug 3, 2007·Journal of Biochemistry and Molecular Biology·Si-Bao WanWei-Dong Huang
Citations
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·Y S KolesnikovV S Kravets
Nov 3, 2011·Lipids in Health and Disease·Slim Abdelkafi, Abdelkarim Abousalham
Apr 22, 2014·Nitric Oxide : Biology and Chemistry·Georgina Corti MonzónLaura de la Canal
Dec 5, 2015·Analytical Chemistry·Renaud RahierAbdelkarim Abousalham
Jun 10, 2015·Phytochemistry·Hafeeza KhatoonRenate Ulbrich-Hofmann
Jul 5, 2015·Journal of Plant Physiology·Marcela PinedoLaura de la Canal
Jan 20, 2017·BioMed Research International·Renaud RahierAbdelkarim Abousalham
May 15, 2021·Critical Reviews in Biotechnology·Peng ZhangJin-Song Shi