PMID: 506Jan 1, 1975

Phospholipases. III. Effects of ionic surfactants on the phospholipase-catalyzed hydrolysis of unsonicated egg lecithin liposomes

The Journal of Membrane Biology
A R GoldhammerE H Cordes

Abstract

Apparent values of Km and Vmax have been measured for catalysis of hydrolysis of unsonicated egg lecithin liposomes, activated through addition of 0.4 M n-hexanol, by phospholipases A2 from bee and snake venoms and by phospholipase C from Clostridium welchii as a function of the concentration of three surfactants: hexadecylamine, hexadecyltrimethylammonium bromide, and dihexadecyl phosphate. For all three enzymes, values of Km and Vmax show little or no dependence on the concentration of these ionic surfactants, demonstrating that the liposomal surface charge is not a crucial factor in determining susceptibility to phospholipase-catalyzed hydrolysis.

References

Jan 1, 1972·Annual Review of Biochemistry·J W Hinman
Mar 15, 1973·FEBS Letters·S GattY Barenholz
Jan 1, 1968·Progress in Biophysics and Molecular Biology·A D BANGHAM
Jan 1, 1971·Biochimie·P Desnuelle
Jun 29, 1971·European Journal of Biochemistry·R A ShipoliniB E Banks
Dec 1, 1971·Chemistry and Physics of Lipids·G ZografiG H de Haas
Jun 4, 1968·Biochimica Et Biophysica Acta·B Entressangles, P Desnuelle
Jan 1, 1965·Journal of the American Oil Chemists' Society·W S SINGLETONJ L WHITE
Dec 1, 1972·The Journal of Membrane Biology·M Montal

Citations

Oct 20, 1976·The Journal of Membrane Biology·R M DAWSONA D BANGHAM
Jan 1, 1987·The Journal of Membrane Biology·Y Kimura
Sep 25, 1992·Biochemical Pharmacology·W PruzanskiP Vadas
May 16, 1989·Biochemistry·M K JainM H Gelb
Sep 1, 1987·Digestive Diseases and Sciences·C H KimA R Zinsmeister
Oct 30, 1978·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·K KellerB Zimmermann

Related Concepts

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