Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization

Lab on a Chip
Bo HuangRichard N Zare

Abstract

Polydimethylsiloxane (PDMS) surfaces can be functionalized with biotin groups by adding biotinylated phospholipids to the PDMS prepolymer before curing. The addition of beta-D-dodecyl-N-maltoside (DDM) in the solution blocks non-specific protein binding on these functionalized PDMS surfaces. We characterize the surface by measuring fluorescently labeled streptavidin binding. Single molecule tracking shows that the phospholipids are not covalently linked to PDMS polymer chains, but the surface functionalization is not removed by washing. We demonstrate the immobilization of biotinylated antibodies and lectins through biotin-avidin interactions.

References

Sep 19, 2002·Analytical Chemistry·Rebecca J WhelanRichard N Zare
Oct 4, 2002·Chembiochem : a European Journal of Chemical Biology·Lars NeumannBrian K Kobilka
May 2, 2003·Analytical Chemistry·Jörg LahannRobert Langer
Oct 16, 2003·The Journal of Biological Chemistry·Gayathri SwaminathBrian K Kobilka
Nov 13, 2003·Electrophoresis·Honest MakambaJong Hoon Hahn
Apr 1, 2004·Analytical Chemistry·Matthew A HoldenPaul S Cremer
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hongkai WuRichard N Zare

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Citations

Mar 14, 2008·Journal of Neurophysiology·S R LockeryB L Pruitt
Apr 28, 2011·Biomicrofluidics·Huei-Wen WuGwo-Bin Lee
Aug 18, 2010·Lab on a Chip·Mairi E SandisonAndrew R Pitt
Feb 19, 2010·Journal of Colloid and Interface Science·Kanaka Hettiarachchi, Abraham P Lee
Jun 24, 2009·Biomaterials·Christopher MoraesCraig A Simmons
Apr 4, 2008·Electrophoresis·Hui YanHongkai Wu
Dec 31, 2010·Colloids and Surfaces. B, Biointerfaces·Maria-Elena VlachopoulouSotirios E Kakabakos
Jun 6, 2020·Annual Review of Biomedical Engineering·Erica A CastilloBeth L Pruitt

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