Phospholipid concentration in lung lavage fluid as biomarker for pulmonary fibrosis

Inhalation Toxicology
Kaori KurodaIsamu Tanaka

Abstract

Pulmonary surfactant comprised primarily of phospholipids is a phospholipid-protein complex synthesized by type II alveolar epithelial cells or Clara cells and secreted to the pulmonary alveoli. As changes have been found in phospholipid concentrations in the bronchoalveolar lavage fluid (BALF) of patients with pulmonary fibrosis, phospholipid is considered to be involved in the process of fibrois/fibrotic process. Therefore, we made a crystalline silica rat model and measured phospholipid concentrations in lung lavage fluid in order to study the relationship of phospholipid to particle-induced pulmonary fibrosis. Eight-week-old Wistar male rats (n = 35) were injected with 2 mg crystalline silica particles suspended in 0.4 ml physiological saline. Rats in the control group (n = 35) were injected with physiological saline only. There were 7 rats in each of the ten subgroups. Rats were sacrificed and dissected at 3 days, 1 wk, 1 mo, 3 mo, and 6 mo after injection. Bronchoalveolar lavage was conducted on bronchoalveoli recovered from the right lung of each rat, the lavage fluid was centrifuged, and the supernatant was used to measure phospholipid concentration. The results were compared with previously reported inflammation scores...Continue Reading

References

Jan 1, 1992·The Journal of Clinical Investigation·P E ZimmermanW J Martin
Mar 1, 1988·The American Review of Respiratory Disease·P C RobinsonR J Mason
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·T HoriuchiR J Mason
Feb 2, 1999·American Journal of Respiratory and Critical Care Medicine·A BergerL Touqui
Apr 9, 1999·The Journal of Clinical Investigation·A M LeVineT Korfhagen
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·P BorronJ R Wright
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Young Ki KimChung Soo Lee
Sep 30, 2003·Inhalation Toxicology·Bernd BellmannJuan M Riego Sintes

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