PMID: 3758474Oct 1, 1986Paper

Phospholipid-dependent Ca2+-activated protein kinase (C-kinase) in the pituitary: further characterization and endogenous redistribution

Molecular and Cellular Endocrinology
J HermonZ Naor

Abstract

Phospholipid-dependent, Ca2+-activated protein kinase (C-kinase) was recently shown to be expressed in rat pituitary. The enzyme is activated by Ca2+ and phosphatidylserine (PS). Diacylglycerol (DG), which is liberated during phosphoinositide turnover, and the potent tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activate pituitary C-kinase in the presence of PS, even at resting levels of intracellular Ca2+ (10(-7) M), and increase the apparent affinity of the enzyme for Ca2+. While micromolar concentration of Ca2+ had no effect on the apparent affinity of the enzyme for PS (Km approximately 15 micrograms/ml), elevation of Ca2+ to the millimolar range produced a sharp increase in the apparent affinity for PS (Km approximately 5 micrograms/ml). Elevation of PS (up to 500 micrograms/ml) could not replace Ca2+ in supporting maximal enzyme activity even in the presence of DG. Cytosolic pituitary C-kinase (70% of total enzyme activity) is recovered in an inactive state and can be activated without further purification. The particulate enzyme (30%) is recovered in a cofactors-insensitive form but can be activated after detergent-solubilization and anion exchange chromatography. Endogenous redistribution of soluble pituita...Continue Reading

References

Jan 13, 1978·Science·P Greengard
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E MelloniB L Horecker
Jul 31, 1985·Biochemical and Biophysical Research Communications·Y UratsujiY Nishizuka
Jul 31, 1985·Biochemical and Biophysical Research Communications·Z Naor, Y Eli
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Z NaorJ Hermon
May 1, 1984·Molecular and Cellular Endocrinology·J F KuoG J Mazzei
Feb 1, 1984·Molecular and Cellular Endocrinology·J L TurgeonD A Walsh
Jul 18, 1984·Biochemical and Biophysical Research Communications·P M Tapley, A W Murray

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