PMID: 9178424Mar 1, 1997Paper

Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: the effects of a peripheral protein on collective lipid fluctuations

Solid State Nuclear Magnetic Resonance
T J PinheiroA Watts

Abstract

The dynamics of the glycerol headgroup of dioleoylphosphatidylglycerol (DOPG) in hydrated bilayers were studied by 2H and 31P NMR spectroscopy, and the effects of binding a peripheral protein, cytochrome c, were evaluated. The fast headgroup segmental motions (tau c, 10(-10)-(-13) s) of DOPG in fully hydrated bilayers were not affected upon binding of cytochrome c, as evaluated by the spin-lattice (T1) relaxation of deuterons in the DOPG glycerol headgroup. In contrast, the spin-spin (T2e) relaxation is strongly affected, indicating that slow cooperative bilayer motions (tau c, 10(-3)-10(-6) s) are enhanced upon the interaction with cytochrome c, 2H and 31P NMR spectral lineshape analysis reveal details of the nature of these motions. The importance of these effects are discussed in terms of a possible mechanism for modulating membrane-associated processes.

References

Aug 1, 1992·Biophysical Journal·R SmithB A Cornell
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P I WatnickS I Chan
Nov 18, 1980·Biochimica Et Biophysica Acta·B de Kruijff, P R Cullis
Nov 17, 1980·Biochemical and Biophysical Research Communications·S B Farren, P R Cullis

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