PMID: 2505213Jun 1, 1989Paper

Phospholipid metabolism in Plasmodium-infected erythrocytes: guidelines for further studies using radioactive precursor incorporation

Parasitology
H J VialJ R Philippot

Abstract

The biosynthesis of phospholipids is extensive in Plasmodium knowlesi-infected simian erythrocytes due to the synthesis of membranes by this single-cell eukaryote in a host erythrocyte devoid of any pathway for lipid biosynthesis. In the present paper, we show that the incorporation of [3H]glycerol, which reflects de novo biosynthesis, is better studied at 300 microM-1 mM than at the trace doses, since this non-physiological precursor does not modify the amount of phosphatidylcholine biosynthesis from [3H]choline. Time-course incorporation of radioactive glycerol, oleate, lysophosphatidylcholine, choline, and inositol in RPMI 1640 medium containing nutrients for lipid synthesis showed that the optimum incubation time for phospholipid studies is 60-90 min, after which radioactive incorporation slows considerably. On the other hand, studies with [14C]serine revealed that incubation for 2-3 h is necessary for isotopic labelling of phosphatidylcholine via phosphatidylserine decarboxylation and phosphatidylethanolamine N-methylation. Incorporation of the various fatty acids into individual lipids was related to the molecular species composing each of them. Studies with [14C palmitoyl] lysophosphatidylcholine showed a very fast intra...Continue Reading

References

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Citations

May 22, 2004·European Journal of Biochemistry·Alicia S CoutoRosa Erra-Balsells
May 19, 2018·PLoS Pathogens·Dora PosfaiEmily R Derbyshire
May 27, 2008·Nature Structural & Molecular Biology·Zachary E R NewbyRobert M Stroud
Sep 13, 1990·Nature·R H Goodwin
Feb 25, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Hugues AhibohDagui Monnet
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dominique PromeneurNirbhay Kumar
Jun 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R H Goodwin
May 30, 2002·Molecular and Biochemical Parasitology·Eric D Lund, Fu-Lin E Chu
Jan 1, 1993·Progress in Lipid Research·J D Smith
Jul 6, 2010·International Journal for Parasitology·Sandrine DéchampsHenri J Vial

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