PMID: 9182701Jun 1, 1997Paper

Phospholipid metabolism of serine in Plasmodium-infected erythrocytes involves phosphatidylserine and direct serine decarboxylation

The Biochemical Journal
N ElabbadiH J Vial

Abstract

Erythrocytes infected with Plasmodium falciparum or Plasmodium knowlesi efficiently incorporated radioactive serine into phosphatidylserine (PtdSer), phosphatidylethanolamine (PtdEtn) and phosphatidylcholine (PtdCho). Serine was also metabolized into ethanolamine (Etn) and phosphorylethanolamine (P-Etn) via direct serine decarboxylation; this is a major phenomenon since together these metabolites represent 60% of total radioactive water-soluble metabolites. They were identified by reverse-phase HPLC and two TLC-type analyses and confirmed by alkaline phosphatase treatment, which depleted the radioactive P-Etn peak completely with a concomitant increase in that of Etn. In the presence of 5 microM labelled serine, radioactivity appeared in Etn and P-Etn after a 25 min lag period, and isotopic equilibrium was reached at 40 and 95 min respectively. There was a similar lag period for PtdEtn formation, which accumulated steadily for at least 180 min. Incorporation of serine into phospholipids and water-soluble metabolites increased in the presence of up to 500 microM external serine. An apparent plateau was then reached for all metabolites except intracellular serine and Etn. Exogenous Etn (at 20 microM) induced a concomitant dramati...Continue Reading

Citations

Nov 15, 2003·Cell Death and Differentiation·K S LangT Wieder
Mar 17, 2004·The Journal of Biological Chemistry·Vladislav DolgachevDuska Separovic
Sep 22, 2006·Infection and Immunity·Françoise Debierre-GrockiegoRalph T Schwarz
Mar 12, 2008·Antimicrobial Agents and Chemotherapy·Christoph GelhausMatthias Leippe
Jun 30, 2009·Journal of Lipid Research·Sandrine DéchampsHenri J Vial
Jun 19, 2010·Drug Development Research·Choukri Ben MamounHenri Vial
Oct 6, 2018·The Journal of Biological Chemistry·Nicole KilianChoukri Ben Mamoun
Jun 18, 2004·The Journal of Biological Chemistry·Michael C KerstingGeorge M Carman
Jul 20, 2001·The Journal of Biological Chemistry·D RonteinA D Hanson
Nov 5, 2011·Assay and Drug Development Technologies·Pulin CheQianjun Li
Jun 26, 2015·Open Forum Infectious Diseases·Izabella SurowiecJohan Normark
Aug 26, 2003·Antimicrobial Agents and Chemotherapy·Dmitri E FomenkoInessa A Khmel
Mar 28, 2007·Applied Microbiology and Biotechnology·Elinor ScottJohan Sanders
Apr 11, 2012·International Journal of Molecular Sciences·Yerim KwonByeong-Ha Lee
Mar 23, 2006·The Journal of Biological Chemistry·Michael C Kersting, George M Carman
Apr 8, 2016·International Journal for Parasitology. Drugs and Drug Resistance·William H WitolaMark McHugh
Apr 6, 1999·Pharmacology & Therapeutics·P L Olliaro, Y Yuthavong
May 30, 2002·Molecular and Biochemical Parasitology·Eric D Lund, Fu-Lin E Chu
Mar 5, 2003·Molecular and Biochemical Parasitology·Henri J VialJaap J van Hellemond
May 22, 2003·Microbes and Infection·Toshihide Mitamura, Nirianne Marie Q Palacpac
Apr 14, 2010·International Journal for Parasitology·Shilpa ShastriHenri J Vial
Jul 6, 2010·International Journal for Parasitology·Sandrine DéchampsHenri J Vial

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