PMID: 3755359Jun 3, 1986Paper

Phospholipid packing asymmetry in curved membranes detected by fluorescence spectroscopy

Biochemistry
J Bramhall

Abstract

There are distinct differences in the molecular packing of phospholipid molecules in the inner and outer membrane monolayers of small lipid vesicles; a small radius of curvature imparts an asymmetry to the interface between these two monolayers. I have used an amphiphilic fluorescent probe, N-[5-(dimethylamino)naphthalenyl-1-sulfonyl]glycine (dansylglycine), to determine if this asymmetry in molecular packing leads to the existence of different environments for fluorescent probes resident in the membrane. Dansylglycine is highly sensitive to the dielectric constant of its environment, and the fluorescence signal from membrane-bound dye is distinct from that in the aqueous medium. When dansylglycine is first mixed with vesicles, it rapidly partitions into the outer monolayer; the subsequent movement of dye into the inner monolayer is much slower. Because of the time lag between the initial partitioning and the subsequent translocation, it is possible to measure the emission spectrum from membrane-bound dye before and after translocation, thus distinguishing the two potential environments for dansylglycine molecules. In the outer membrane monolayer of small dipalmitoylphosphatidylcholine vesicles, dye fluorescence emission is max...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C Huang, J T Mason
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Apr 26, 1985·Biochimica Et Biophysica Acta·B LeeJ Bramhall
Apr 1, 1969·Chemistry and Physics of Lipids·M D BarrattD Chapman
Nov 21, 1972·Biochemistry·M P Sheetz, S I Chan
Dec 19, 1984·Biochimica Et Biophysica Acta·J Bramhall
Apr 2, 1982·Science·S A SimonR Latorre
Sep 9, 1982·Biochimica Et Biophysica Acta·F Jähnig, J Bramhall
Jul 1, 1956·Biochimica Et Biophysica Acta·B S HARTLEY, V MASSEY

❮ Previous
Next ❯

Citations

Sep 1, 1987·Biophysical Chemistry·M Brullemans, P Tancrde
May 16, 2000·Chemistry and Physics of Lipids·R M Epand, R Kraayenhof
Aug 26, 2009·Journal of the American Chemical Society·Hyun Soo LeePeter G Schultz
Sep 26, 2008·The Journal of Physical Chemistry. B·Xuelian LiPunit Kohli
Aug 12, 2014·The Journal of Chemical Physics·Hung-Yu ChangHeng-Kwong Tsao
Feb 14, 2006·Biochimica Et Biophysica Acta·Satoshi OhtakeJuan J de Pablo
Nov 23, 2017·Photochemistry and Photobiology·Masahiko Taniguchi, Jonathan S Lindsey
May 11, 2004·Journal of Combinatorial Chemistry·G Pina-LuisI A Rivero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.