Phospholipid-templated silica nanocapsules as efficient polyenzymatic biocatalysts

Dalton Transactions : an International Journal of Inorganic Chemistry
Lai Truong PhuocAnne Galarneau

Abstract

Solid polyenzymatic biocatalysts have been designed by combining two immobilized enzymes, the first one allowing the in situ generation of H(2)O(2) from air and the second one performing an oxidation reaction. The in situ H(2)O(2) generation system is based on the reaction of glucose with air using a glucose oxidase (GOx). The optimization of the encapsulation of GOx into phospholipids-templated silica capsules (NPS) was performed. A bienzymatic system made of GOx and horseradish peroxidase (HRP) was studied. Optimal conditions for the activity of the GOx/HRP bienzymatic system have been determined for both homogeneous and heterogeneous conditions. The encapsulation in NPS materials increases the stability of both enzymes. The performance of the encapsulated bienzymatic GOx/HRP system in the model reaction of 4-aminoantipyridine with phenol is similar when the enzymes are immobilized separately in two NPS or coencapsulated in the same NPS. An excess of peroxidase compared to GOx ([HRP]/[GOx] = 5-10) is necessary to obtain the optimal activity. To show the potentiality of bienzymatic systems in real applications, HRP has been replaced by hemoglobin, which is known for its ability to oxidize polycyclic aromatic hydrocarbons (PAH)...Continue Reading

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Citations

Jul 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Antonio RanieriElena Castellini
Apr 10, 2012·Journal of Hazardous Materials·Jiayin LiuZhengqiang Li
Mar 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dorothee I FriedMichael Fröba
Mar 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Thomas MoreauThibaud Coradin

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