PMID: 6975121Sep 15, 1981Paper

Phospholipid topography of the photosynthetic membrane of Rhodopseudomonas sphaeroides

Biochemistry
K K Al-Bayatti, J Takemoto

Abstract

The topography of phospholipids in the photosynthetic membranes of Rhodopseudomonas sphaeroides was investigated by using purified chromatophores and spheroplast-derived vesicles (SDVs). Chromatophores are closed vesicles oriented inside out with respect to the cytoplasmic membrane (cytoplasmic side out) and obtained from French-pressed cell lysates. SDVs are oriented right side out (periplasmic side out) and are obtained after osmotic lysis of lysozyme-treated cells. Phosphatidylethanolamine (PE) comprised approximately 62% and phosphatidylglycerol (PG) comprised approximately 33% of the total phospholipid of both vesicle preparations. The relatively membrane impermeable reagent trinitrobenzenesulfonate (TNBS) at 3 mM concentration and 5 degrees C modified chromatophore and SDV PE with kinetics indicating the occurrence of fast- and slow-reacting pools of PE. The fast-reacting pools comprised 33% and 55% of the total PE of chromatophores and SDVs, respectively. The slow-reacting pools comprised 61% and 32% of the total PE of chromatophores and SDVs, respectively. Phospholipase A2 treatment of chromatophores (1 unit/mg of vesicle protein) for 1 h at 37 degrees C resulted in hydrolysis of 73% and 77% of the total PG and PE, resp...Continue Reading

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Citations

Jun 16, 1983·Biochimica Et Biophysica Acta·K Tsuchihashi, O Minari
Dec 2, 2009·Biophysical Journal·Danielle E ChandlerKlaus Schulten
Jun 5, 2014·Biophysical Journal·Danielle E ChandlerKlaus Schulten
May 6, 2016·Annual Review of Biophysics·Boon Chong GohKlaus Schulten

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