PMID: 3768371Nov 6, 1986Paper

Phospholipid topology and flip-flop in intestinal brush-border membrane

Biochimica Et Biophysica Acta
L I BarsukovG Semenza

Abstract

The topological distribution of the two major phospholipids of brush-border membrane, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), has been investigated using brush-border membrane vesicles from rabbit small intestine. Bee venom phospholipase A2 and phosphatidylcholine exchange protein from bovine liver were used as membrane probes. It is shown that the brush-border membrane retains its integrity under conditions of phospholipase hydrolysis and intermembrane phospholipid exchange. Kinetic analysis of the data of phospholipase hydrolysis and phospholipid exchange at temperatures under 10 degrees C shows that both PC and PE occur in two pools: a minor (about 25%) more readily accessible pool and a major one (about 75%) less readily available. The rate of PC exchange between these two pools is relatively fast. The half-time derived under conditions of phospholipase hydrolysis is of the order of 20 min. Under conditions of phospholipid exchange the exchange rates may be even faster. The difference in exchange kinetics observed with the two methods of probing is probably due to changes in membrane properties such as the bilayer fluidity induced by the probing process itself. It is proposed that the two pools represent...Continue Reading

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Citations

Jun 15, 1990·Experientia·A Zachowski, P F Devaux
Jun 6, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kenjiro KogaKanji Takada
Aug 23, 1988·Biochemistry·K Simons, G van Meer
Feb 25, 2005·American Journal of Physiology. Cell Physiology·Warren G HillMark L Zeidel
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
Jul 7, 1988·Biochimica Et Biophysica Acta·C Venien, C Le Grimellec
Nov 13, 1991·Biochimica Et Biophysica Acta·P Proulx
Mar 23, 1992·Biochimica Et Biophysica Acta·M KobayashiK Miyazaki
Mar 18, 1991·Biochimica Et Biophysica Acta·F TacnetP Ripoche

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