Phospholipids containing ether-bound hydrocarbon-chains are essential for efficient phagocytosis and neutral lipids of the ester-type perturb development in Dictyostelium.

Biology Open
Frederik KappeltMarkus Maniak

Abstract

Lipids are the building blocks for cellular membranes; they provide signalling molecules for membrane dynamics and serve as energy stores. One path of their synthesis is initiated by glycerol-3-phosphate acyltransferase (GPAT), which in Dictyostelium resides on the endoplasmic reticulum. When an excess of fatty acids is present, it redistributes to storage organelles, the lipid droplets. Mutants, where the GPAT was eliminated by homologous recombination, produce fewer lipid droplets and are almost devoid of triacylglycerols (TAG), rendering them more resistant to cell death and cell loss in the developmental stages preceding fruiting body formation. The enzyme most closely related to GPAT is called FARAT, because it combines a fatty acyl-reductase (FAR) and an acyltransferase (AT) domain in its sequence. The protein is confined to the lumen of the peroxisome, where it transfers a fatty acid to dihydroxyacetone-phosphate initiating the synthesis of ether lipids, later completed at the endoplasmic reticulum. A mutant lacking FARAT produces lipid droplets that are devoid of the storage lipid monoalkyl-diacyl-glycerol (MDG), but the efficiency of spore formation in the developmental cycle is largely unaltered. Instead, these mutant...Continue Reading

References

Sep 25, 1987·Journal of Chromatography·C LerayJ P Cazenave
Mar 29, 1985·Biochimica Et Biophysica Acta·B Z Ngwenya, N Yamamoto
Jan 27, 1995·Cell·N Divecha, R F Irvine
Sep 6, 2000·Developmental Medicine and Child Neurology·L SztrihaG G Lestringant
Jun 14, 2002·Cell·Miho Iijima, Peter Devreotes
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Dec 2, 2004·Journal of Cell Science·Dirk DormannCornelis J Weijer
May 6, 2005·Nature·L EichingerA Kuspa
Jan 27, 2006·Journal of Lipid Research·Laurent VergnesKaren Reue
Feb 2, 2006·Journal of Lipid Research·Anne P BeigneuxStephen G Young
Jun 6, 2006·European Journal of Cell Biology·Dirk WienkeMarkus Maniak
Sep 8, 2006·Methods in Molecular Biology·Francisco Rivero, Markus Maniak
Sep 14, 2006·Journal of Cell Science·Valentina MercantiPierre Cosson
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jingsong CaoRuth E Gimeno
Mar 9, 2007·Traffic·Harriët M LooversPeter J M van Haastert
Apr 28, 2007·Current Biology : CB·Oliver Hoeller, Robert R Kay
Feb 2, 2008·The Journal of Biological Chemistry·Yan Qun ChenGuoqing Cao
Apr 2, 2009·American Journal of Physiology. Endocrinology and Metabolism·Kazuharu Takeuchi, Karen Reue
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Barbara BalestrieriJonathan P Arm
Sep 22, 2009·Traffic·Johanna SpandlChristoph Thiele
Apr 29, 2010·The Journal of Biological Chemistry·Prakash G ChandakDagmar Kratky
Aug 9, 2011·Biochimica Et Biophysica Acta·Karlheinz GrillitschGünther Daum
Jul 4, 2012·Journal of Leukocyte Biology·Kenneth R FeingoldCarl Grunfeld
Jul 12, 2013·Journal of Cell Science·Oliver HoellerRobert R Kay

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
PCR
confocal microscopy
Enzymatic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.