PMID: 9449129Feb 4, 1998Paper

Phosphonates as 31P-NMR markers of extra- and intracellular space and pH in perfused rat liver

NMR in Biomedicine
K BruynseelsF Vanstapel

Abstract

We evaluated phosphonates (Po) as markers of the extra- and intracellular space in perfused rat liver. (i) In- and outwash behaviour of phenylphosphonate (PhePo), 3-amino-propylphosphonate (NProPo) and methyl phosphonate (MePo) was compared with that of creatine phosphate (CrP), a marker of the extracellular space, and of dimethyl methylphosphonate (MePoMe2), a marker of the total water-accessible space. In- and outwash of CrP was accurately predicted by the time constant (approximately 12 s) for the in- and outwash of inulin, a standard marker of the extracellular space. MePoMe2 rapidly distributed over the total liver volume (about three times the CrP accessible space). PhePo, NProPo and MePo washed rapidly into the extracellular space with CrP, and then steadily spilled over into the MePoMe2-accessible space. Upon outwash, Po signals rapidly declined in phase with that of CrP. Residual Po (PhePo > NProPo approximately equal to MePo) reflected the amount internalized during prolonged (60 min) inwash. Proportional amounts of residual Po were found in extracts of livers harvested after outwash of perfusate and extracellular markers. Consistent with exclusion from the cells, CrP went undetected in these extracts. (ii) The resona...Continue Reading

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Citations

May 8, 2004·Antioxidants & Redox Signaling·Valery V KhramtsovDavid J Lurie
Nov 26, 2016·Chembiochem : a European Journal of Chemical Biology·Sophie Thétiot-LaurentSylvia Pietri

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