Phosphoprotein extraction from the dentine/cementum complex of human tooth roots

Archives of Oral Biology
S P McCurdyF F Feagin

Abstract

Root shards were placed in dialysis tubing and demineralized to completion in either 10% disodium EDTA, pH 7.4, 0.6 M HCl, 0.1 M HCl, 0.5 M acetic or 75 mM-25 mM lactic-acetic acids. The demineralized shards were then re-extracted with 0.05 M tris-HCl, 1.0 M NaCl. DEAE chromatography revealed that the major peak of the 0.6 M CHl and EDTA extracts contained organic phosphorus, whereas much less organic phosphorus was found in the major peak of the 0.1 M HCl extract. Analysis of the re-extracts gave a pattern opposite to that obtained from the initial extractions. Measurements of protein and organic phosphorus released during extraction and re-extraction confirmed these results. Staining of SDS-PAGE gels for phosphoprotein with Stains-All resulted in a blue smear in fractions containing organic phosphorus. Thus the extraction of phosphoproteins from human tooth roots differed depending upon the demineralizing conditions. This ability to remove phosphoprotein differentially will allow further investigation of the role of phosphoprotein in mineralization and remineralization.

References

Jan 1, 1977·Archives of Oral Biology·M Thomas, A G Leaver
Jun 28, 1977·Biochemistry·S L LeeT Glonek
Jan 1, 1975·Calcified Tissue Research·C V De Steno, F F Feagin
Nov 24, 1975·Calcified Tissue Research·I R DicksonA Veis
May 1, 1977·Calcified Tissue Research·A G LeaverI B Holbrook
Jan 1, 1986·Connective Tissue Research·Y TakagiS Sasaki
Jan 26, 1972·Biochimica Et Biophysica Acta·W T ButlerC V Desteno
May 1, 1984·Calcified Tissue International·Y Takagi, A Veis
Jan 1, 1983·Calcified Tissue International·P M Masters
Jan 1, 1981·European Journal of Biochemistry·M ZanettiA Linde

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Citations

Sep 1, 1996·Calcified Tissue International·S R ChangB H Clarkson
Apr 1, 1996·Archives of Oral Biology·R M CarvalhoD H Pashley
Dec 1, 1992·Archives of Oral Biology·S P McCurdyF F Feagin
Sep 1, 1993·Archives of Oral Biology·B H ClarksonA R Hand
Jan 1, 1995·Connective Tissue Research·A S NarayananS Pitaru
Jan 1, 1996·Connective Tissue Research·A S Narayanan, P M Bartold
Feb 5, 2014·Journal of Dentistry·Manuel ToledanoRaquel Osorio
Oct 16, 1999·Oral Diseases·T Z Dung, A H Liu
Jul 1, 1992·Bone and Mineral·H ArzateA S Narayanan

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