Phosphoproteome characterization reveals that Sendai virus infection activates mTOR signaling in human epithelial cells

Proteomics
Tiina ÖhmanTuula A Nyman

Abstract

Sendai virus (SeV) is a common respiratory pathogen in mice, rats, and hamsters. Host cell recognition of SeV is mediated by pathogen recognition receptors, which recognize viral components and induce intracellular signal transduction pathways that activate the antiviral innate immune response. Viruses use host proteins to control the activities of signaling proteins and their downstream targets, and one of the most important host protein modifications regulated by viral infection is phosphorylation. In this study, we used phosphoproteomics combined with bioinformatics to get a global view of the signaling pathways activated during SeV infection in human lung epithelial cells. We identified altogether 1347 phosphoproteins, and our data shows that SeV infection induces major changes in protein phosphorylation affecting the phosphorylation of almost one thousand host proteins. Bioinformatics analysis showed that SeV infection activates known pathways including MAPK signaling, as well as signaling pathways previously not linked to SeV infection including Rho family of GTPases, HIPPO signaling, and mammalian target of rapamycin (mTOR)-signaling pathway. Further, we performed functional studies with mTOR inhibitors and siRNA approac...Continue Reading

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Citations

Jan 18, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shanshan XingHong Yin
Aug 4, 2016·Molecular & Cellular Proteomics : MCP·Sandra SöderholmTuula A Nyman
Jan 10, 2018·Frontiers in Cellular and Infection Microbiology·Jeffrey M Grabowski, Catherine A Hill
Oct 6, 2020·Frontiers in Immunology·Tünde FeketeKitti Pázmándi
Jan 21, 2021·Experimental Biology and Medicine·Suresh MishraBl Grégoire Nyomba

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