Phosphoproteome reveals an atlas of protein signaling networks during osteoblast adhesion

Journal of Cellular Biochemistry
Renato MilaniWillian F Zambuzzi

Abstract

Cell adhesion on surfaces is a fundamental process in the emerging biomaterials field and developmental events as well. However, the mechanisms regulating this biological process in osteoblasts are not fully understood. Reversible phosphorylation catalyzed by kinases is probably the most important regulatory mechanism in eukaryotes. Therefore, the goal of this study is to assess osteoblast adhesion through a molecular prism under a peptide array technology, revealing essential signaling proteins governing adhesion-related events. First, we showed that there are main morphological changes on osteoblast shape during adhesion up to 3 h. Second, besides classical proteins activated upon integrin activation, our results showed a novel network involving signaling proteins such as Rap1A, PKA, PKC, and GSK3beta during osteoblast adhesion on polystyrene. Third, these proteins were grouped in different signaling cascades including focal adhesion establishment, cytoskeleton rearrangement, and cell-cycle arrest. We have thus provided evidence that a global phosphorylation screening is able to yield a systems-oriented look at osteoblast adhesion, providing new insights for understanding of bone formation and improvement of cell-substratum i...Continue Reading

References

Jan 1, 1978·International Review of Cytology·F Grinnell
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R O Stuart, S K Nigam
Aug 1, 1995·Experimental Cell Research·M Nilsson, L E Ericson
May 5, 1995·The Journal of Biological Chemistry·D L AltschulerE G Lapetina
Jun 1, 1994·The Journal of Investigative Dermatology·J E LewisM J Wheelock
Oct 1, 1993·The Journal of Cell Biology·M S BaldaJ M Anderson
Jan 15, 1997·The EMBO Journal·B FrankeJ L Bos
Jan 7, 2000·Biochemical and Biophysical Research Communications·S DremierC Maenhaut
Dec 10, 2002·Science·G ManningS Sudarsanam
Jul 1, 1996·Trends in Cell Biology·G I WelshC G Proud
Jul 13, 2004·Biochimica Et Biophysica Acta·Alan K Howe
Sep 10, 2004·The Journal of Biological Chemistry·Sander H DiksMaikel P Peppelenbosch
Aug 23, 2007·PloS One·Sander H DiksMaikel P Peppelenbosch
Oct 16, 2007·Cancer Letters·Karla Cristiana de Souza QueirozCarmen Veríssima Ferreira
Sep 6, 2008·Chemico-biological Interactions·Angelo de FátimaRonaldo A Pilli
Nov 8, 2008·Molecular and Cellular Biochemistry·Willian F ZambuzziCarmen V Ferreira
Dec 18, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Willian F ZambuzziCarmen V Ferreira
Feb 26, 2009·Methods in Molecular Biology·Kaushal ParikhTita Ritsema
May 9, 2009·EMBO Reports·Kiran S NadellaLawrence S Kirschner
Jun 30, 2009·Journal of Cellular Biochemistry·Willian Fernando ZambuzziCarmen Veríssima Ferreira

❮ Previous
Next ❯

Citations

May 24, 2011·Molecular Biotechnology·Alexandra ThieleMike Schutkowski
Jan 26, 2011·Development, Growth & Differentiation·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira
Jan 1, 2012·Scientifica·Maikel P Peppelenbosch
Mar 23, 2011·Journal of Materials Science. Materials in Medicine·Thais Accorsi-MendonçaJosé Mauro Granjeiro
Oct 15, 2013·Journal of Cellular Biochemistry·Gustavo V O FernandesWillian F Zambuzzi
Feb 26, 2011·Biotechnology and Bioengineering·Willian F ZambuzziJosé M Granjeiro
Jul 14, 2010·Journal of Cellular Biochemistry·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira
Mar 12, 2011·Journal of Biomedical Materials Research. Part a·Willian F ZambuzziHiroshi Aoyama
Jan 10, 2012·Clinical Oral Implants Research·Igor Iuco Castro-SilvaJosé Mauro Granjeiro
Sep 8, 2011·Journal of Cellular Physiology·Valquíria P MedeirosHelena B Nader
Mar 29, 2014·Biotechnology and Bioengineering·Sara Gemini-PiperniWillian Zambuzzi
Jun 24, 2017·Journal of Biomedical Materials Research. Part a·M C RossiW F Zambuzzi
Mar 28, 2019·Journal of Cellular Biochemistry·Célio Jr da Costa FernandesWillian F Zambuzzi
Jul 11, 2020·Advanced Biosystems·Célio Jr da Costa FernandesWillian F Zambuzzi
Sep 25, 2017·Journal of Biomedical Materials Research. Part a·Célio J C FernandesWillian F Zambuzzi
Jun 5, 2017·Molecular and Cellular Biochemistry·Celio J da Costa FernandesWillian F Zambuzzi
Oct 30, 2020·Frontiers in Bioengineering and Biotechnology·Marcel Rodrigues FerreiraWillian Zambuzzi
Nov 8, 2020·Journal of Materials Science. Materials in Medicine·Carolina Simão AlbanoPaulo Noronha Lisboa-Filho
Apr 8, 2017·Journal of Cellular Biochemistry·Alessandra V S FariaCarmen V Ferreira-Halder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.