Phosphoproteomics

Current Protocols in Protein Science
Jun ZhongAkhilesh Pandey

Abstract

Protein phosphorylation is one of the most important mechanisms of regulating protein function in cells, and it plays an important role in controlling diverse biological processes, including cellular proliferation, migration, and metabolism. The term "phosphoproteome" refers to the complement of proteins that undergoes phosphorylation, the extent of their phosphorylation status at the level of individual residues, as well as the dynamics of the phosphorylation events in response to various stimuli. This unit provides methods for enrichment of phosphorylated proteins and peptides using anti-phosphotyrosine antibodies or titanium dioxide, respectively. Support protocols are provided for two detergent-free cell lysis methods, fractionation of proteins prior to enrichment, and use of stable isotope labeling by amino acids in cell culture (SILAC) method for studying dynamics of phosphorylation events.

References

Jul 29, 1999·Analytical Chemistry·M C Posewitz, P Tempst
Apr 3, 2001·Nature Biotechnology·H ZhouR Aebersold
Mar 5, 2002·Nature Biotechnology·Scott B FicarroForest M White
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott A GerberSteven P Gygi
Jun 10, 2004·European Journal of Mass Spectrometry·Angela AmoresanoEric Quemeneur
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·John E P SykaDonald F Hunt
Aug 18, 2004·Nature Biotechnology·Blagoy BlagoevMatthias Mann
Sep 4, 2004·Molecular & Cellular Proteomics : MCP·Bryan A BallifSteven P Gygi
Jan 25, 2005·Molecular & Cellular Proteomics : MCP·Albrecht GruhlerOle N Jensen
Apr 29, 2005·Molecular & Cellular Proteomics : MCP·Martin R LarsenThomas J D Jørgensen
Oct 11, 2005·Journal of Proteome Research·Ramars AmanchyAkhilesh Pandey
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Judit VillénSteven P Gygi
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Henrik MolinaAkhilesh Pandey
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·An ChiDonald F Hunt
Feb 13, 2007·Nature Methods·Bernd BodenmillerRuedi Aebersold

❮ Previous
Next ❯

Citations

Dec 6, 2014·Proteomics·Dave C H LeeSimon J Hubbard
Jun 11, 2019·Current Protocols in Protein Science·Aaron R GoldmanDavid W Speicher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.