Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase

FEBS Letters
A TouréJacques Bertoglio

Abstract

MgcRacGAP, a Rho GAP essential to cytokinesis, works both as a Rho GTPase regulator and as a scaffolding protein. MgcRacGAP interacts with MKLP1 to form the centralspindlin complex and associates with the RhoGEF Ect2. The GAP activity of MgcRacGAP is regulated by Aurora B phosphorylation. We have isolated B56epsilon, a PP2A regulatory subunit, as a new MgcRacGAP partner. We report here that (i) MgcRacGAP is phosphorylated by Aurora B and Cdk1, (ii) PP2A dephosphorylates Aurora B and Cdk1 phosphorylated sites and (iii) inhibition of PP2A abrogates MgcRacGAP/Ect2 interaction. Therefore, PP2A may regulate cytokinesis by dephosphorylating MgcRacGAP and its interacting partners.

References

Nov 3, 1995·The Journal of Biological Chemistry·B McCright, D M Virshup
Nov 22, 2000·The Journal of Biological Chemistry·K HiroseT Kitamura
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Feb 20, 2003·The Biochemical Journal·Nathalie NaudGérard Gacon
Apr 12, 2003·Developmental Cell·Yukinori MinoshimaToshio Kitamura
Jun 26, 2003·Nucleic Acids Research·John C ObenauerMichael B Yaffe
Jan 28, 2004·The Journal of Biological Chemistry·Reiko BanHirofumi Tanaka
Apr 16, 2004·Nature·Shingo YasudaShuh Narumiya
Aug 17, 2004·The Journal of Biological Chemistry·Hisayoshi YoshizakiMichiyuki Matsuda
Aug 17, 2005·The Journal of Cell Biology·Ozlem YüceMichael Glotzer
Sep 9, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rym MzaliJacques Bertoglio
Oct 21, 2005·Molecular Biology of the Cell·Keiju KamijoToru Miki
Oct 6, 2006·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara

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Citations

Dec 9, 2008·Nature Cell Biology·Ann L Miller, William M Bement
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Mar CarmenaWilliam C Earnshaw
Nov 19, 2008·Molecular and Cellular Biology·Laetitia SeguinJacques Bertoglio
Sep 3, 2011·PloS One·Caroline LiotJacques Bertoglio
Jul 19, 2012·Annual Review of Cell and Developmental Biology·Rebecca A GreenKaren Oegema
Nov 28, 2012·The Journal of Cell Biology·Mar Carmena
Jan 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nan-Peng ChenElmar Schiebel
Feb 26, 2011·Trends in Cell Biology·K Adam Bohnert, Kathleen L Gould
Aug 25, 2010·Seminars in Cell & Developmental Biology·Max E Douglas, Masanori Mishima
Apr 8, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Stefania CrispiAlessandra Verdina
Feb 23, 2010·IUBMB Life·Stephen L GregoryRobert Saint
Dec 9, 2014·Small GTPases·Sandra CitiJimit Shah
Jul 25, 2012·Cell Cycle·Muriel DavidJacques Bertoglio
May 11, 2010·Current Biology : CB·Max E DouglasMasanori Mishima
Feb 24, 2015·Frontiers in Neuroscience·Roberta AzzarelliEmilie Pacary
Jul 28, 2016·Molecular Cell·Emil Peter Thrane HertzJakob Nilsson
Dec 18, 2013·Genetics·María Alcaide-GavilánJuan Jimenez
Jul 30, 2009·Cancer Research·Armando van der Horst, Kum Kum Khanna
Jun 29, 2011·Journal of Cell Science·Francis A BarrUlrike Gruneberg

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