Phosphorelay of non-orthodox two component systems functions through a bi-molecular mechanism in vivo: the case of ArcB

Molecular BioSystems
Goran JovanovicMichael P H Stumpf

Abstract

Two-component systems play a central part in bacterial signal transduction. Phosphorelay mechanisms have been linked to more robust and ultra-sensitive signalling dynamics. The molecular machinery that facilitates such a signalling is, however, only understood in outline. In particular the functional relevance of the dimerization of a non-orthodox or hybrid histidine kinase along which the phosphorelay takes place has been a subject of debate. We use a combination of molecular and genetic approaches, coupled to mathematical and statistical modelling, to demonstrate that the different possible intra- and inter-molecular mechanisms of phosphotransfer are formally non-identifiable in Escherichia coli expressing the ArcB non-orthodox histidine kinase used in anoxic redox control. In order to resolve this issue we further analyse the mathematical model in order to identify discriminatory experiments, which are then performed to address cis- and trans-phosphorelay mechanisms. The results suggest that exclusive cis- and trans-mechanisms will not be operating, instead the functional phosphorelay is likely to build around a sequence of allosteric interactions among the domain pairs in the histidine kinase. This is the first detailed mec...Continue Reading

References

Jan 1, 1992·Folia Microbiologica·M MáraA Dohnalová
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Apr 4, 2000·Current Opinion in Microbiology·J A Hoch
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Sep 21, 2002·Molecular Biology Reports·B G OlivierJ H S Hofmeyr
Feb 4, 2003·The Journal of Biological Chemistry·Ohsuk KwonEdmund C C Lin
Apr 1, 1963·Journal of Molecular Biology·J MONODF JACOB
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Dec 14, 2007·Annual Review of Genetics·Michael T Laub, Mark Goulian
Jul 7, 2009·Annual Review of Microbiology·Rong Gao, Ann M Stock
Jan 26, 2010·Journal of Bacteriology·Gabriela R Peña-Sandoval, Dimitris Georgellis
Feb 6, 2010·Current Opinion in Microbiology·Kristin WuichetIgor B Zhulin
Mar 13, 2010·Current Opinion in Microbiology·Jonah Cheung, Wayne A Hendrickson
Mar 17, 2010·Current Opinion in Microbiology·Michael Y Galperin
Mar 20, 2010·Molecular BioSystems·Michael Y GalperinEugene Kolker
May 4, 2010·Journal of Molecular Biology·Zhen Zhang, Wayne A Hendrickson
Jul 1, 2010·Science Signaling·Kristin Wuichet, Igor B Zhulin
Aug 13, 2010·Journal of the Royal Society, Interface·Attila Csikász-NagyOrkun S Soyer
Oct 19, 2010·Current Opinion in Structural Biology·Patricia CasinoAlberto Marina
May 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michał KomorowskiMichael P H Stumpf
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chris P BarnesMichael P H Stumpf
Jan 24, 2012·Journal of Theoretical Biology·Michael KnudsenCarsten Wiuf
Mar 2, 2012·Bioinformatics·Michal KomorowskiMichael P H Stumpf
Jul 4, 2012·Annual Review of Microbiology·Emily J Capra, Michael T Laub
Jul 24, 2012·Advances in Experimental Medicine and Biology·Xia ShengMichael P H Stumpf
Sep 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Heather A HarringtonMichael P H Stumpf
Jan 29, 2013·Current Opinion in Microbiology·Anna I Podgornaia, Michael T Laub
Feb 6, 2013·PLoS Computational Biology·Juliane LiepeMichael P H Stumpf
Apr 13, 2013·Current Opinion in Biotechnology·Paul KirkMichael P H Stumpf
Apr 18, 2014·Nature·Hesam N MotlaghVincent J Hilser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.