Phosphorescent soft salt for ratiometric and lifetime imaging of intracellular pH variations

Chemical Science
Yun MaWai-Yeung Wong

Abstract

In contrast to traditional short-lived fluorescent probes, long-lived phosphorescent probes based on transition-metal complexes can effectively eliminate unwanted background interference by using time-resolved luminescence imaging techniques, such as photoluminescence lifetime imaging microscopy. Hence, phosphorescent probes have become one of the most attractive candidates for investigating biological events in living systems. However, most of them are based on single emission intensity changes, which might be affected by a variety of intracellular environmental factors. Ratiometric measurement allows simultaneous recording of two separated wavelengths instead of measuring mere intensity changes and thus offers built-in correction for environmental effects. Herein, for the first time, a soft salt based phosphorescent probe has been developed for ratiometric and lifetime imaging of intracellular pH variations in real time. Specifically, a pH sensitive cationic complex (C1) and a pH insensitive anionic complex (A1) are directly connected through electrostatic interaction to form a soft salt based probe (S1), which exhibits a ratiometric phosphorescent response to pH with two well-resolved emission peaks separated by about 150 nm...Continue Reading

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Citations

Jun 17, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Nong LuPingyu Zhang
Apr 2, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yi LiZong-Wan Mao
Apr 13, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Yun MaWai-Yeung Wong
Jul 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hui WangYupeng Tian
Oct 19, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Kangqiang QiuHui Chao
Jul 22, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Shin-Ya TakizawaShigeru Murata
May 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sabrina ValettiIlse Manet
Dec 19, 2020·Beilstein Journal of Organic Chemistry·Sidharth Thulaseedharan Nair SailajaCristian A Strassert
Dec 9, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Pavel S ChelushkinSergey P Tunik
Dec 9, 2021·Topics in Current Chemistry·Jie ZhangDeman Han

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Methods Mentioned

BETA
imaging microscopy
phosphorescence

Software Mentioned

Fluoview Viewer
Pico Quant
Live Workstation
Kodak Molecular Imaging
SymPhoTime
PLIM

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