Phosphoribosylpyrophosphate (PRPP)-less mutants of Escherichia coli.

Molecular Microbiology
Bjarne Hove-Jensen

Abstract

A DNA fragment encoding kanamycin resistance was inserted in vitro into a plasmid-borne prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli. The resulting plasmids were subsequently transferred to the chromosome by homologous recombination and the haploid strains prs-3::KanR and prs-4::KanR were obtained. These strains were fully viable, but required guanosine, uridine, histidine, tryptophan and nicotinamide mononucleotide. There was no phosphoribosylpyrophosphate synthetase activity or phosphoribosylpyrophosphate pool in the mutant strains. These results show that phosphoribosylpyrophosphate synthetase is dispensable for E. coli.

References

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Citations

Aug 28, 1990·European Journal of Biochemistry·K ArnvigR L Switzer
Nov 25, 2004·European Journal of Biochemistry·Bjarne Hove-Jensen, James N McGuire
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A W Karzai, R T Sauer
Jan 1, 2008·Journal of Microbiological Methods·Bjarne Hove-Jensen
May 22, 2004·Journal of Pharmacological Sciences·Takeshi HiramotoNorihisa Fujita
Dec 30, 2016·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenMartin Willemoës
Aug 4, 2018·Journal of Molecular Endocrinology·Oro UchenunuLaura Hulea
Sep 1, 1993·Journal of Bacteriology·B Hove-Jensen, M Maigaard
Sep 1, 1994·Journal of Bacteriology·L Brøndsted, T Atlung
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs

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