Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina

Biophysical Journal
D V ApteT G Ebrey

Abstract

Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.

Citations

May 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·C T Burt, H Ribolow
Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos

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