Phosphorus, and nitrogen co-doped carbon dots as a fluorescent probe for real-time measurement of reactive oxygen and nitrogen species inside macrophages

Biosensors & Bioelectronics
Yunqian GongXiaoling Zhang

Abstract

Phosphorus and nitrogen doped carbon dots (PN-CDs) were conveniently prepared by carbonization of adenosine-5'-triphosphate using a hydrothermal treatment. The PN-CDs with P/C atomic ratio of ca. 9.2/100 emit blue luminescence with high quantum yields of up to 23.5%. The PN-CDs were used as a novel sensing platform for live cell imaging of reactive oxygen species (ROS) and reactive nitrogen species (RNS), including ClO(-), ONOO(-), and NO in macrophages. The nanosensor design is based on our new finding that the strong fluorescence of the PN-CDs can be sensitively and selectively quenched by ROS and RNS both in vitro and in vivo. These results reveal that the PN-CDs can serve as a sensitive sensor for rapid imaging of ROS and RNS signaling with high selectivity and contrast.

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Citations

Dec 20, 2016·Analytical Chemistry·Sagarika BhattacharyaRaz Jelinek
Sep 27, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan Jun JuNian Bing Li
Mar 21, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ayan PalArun Chattopadhyay
Apr 22, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Xu TengBuhong Li
Sep 25, 2018·Analytical and Bioanalytical Chemistry·Liqiang YanJianping Li
Nov 13, 2018·Nano-micro Letters·Kok Ken ChanKen-Tye Yong
Dec 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Quan XuNeng Li
Oct 18, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Jianfei WeiRui Wang
Jul 3, 2019·Advances in Colloid and Interface Science·Bin Bin ChenCheng Zhi Huang
Jan 6, 2019·Talanta·Manuel AlgarraJosé Jiménez-Jiménez
Apr 20, 2021·Colloids and Surfaces. B, Biointerfaces·Legha AnsariMohammad Amjadi
Apr 27, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Yi YangWei Dong
Aug 28, 2021·Journal of Biomedical Nanotechnology·Xiao-Lin WangHan-Jun Li

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