Phosphorus metabolites in different muscles of the rat leg by 31P image-selected in vivo spectroscopy

NMR in Biomedicine
B MadhuB Soussi

Abstract

The difference in concentration of phosphorylated metabolites in muscles with different fiber composition was studied in vivo by localized 31P nuclear magnetic resonance spectroscopy in the rat hindlimb 120-160 microliters volumes were selected in regions containing the soleus and gastrocnemius muscles. Concentrations of phosphocreatine (PCr), adenosine triphosphate and inorganic phosphate (Pi) were determined and intracellular pH was calculated in the respective muscle groups. The highest level of PCr was found in the gastrocnemius muscle, containing 30.7 mmoles/dm3 tissue compared to 22.3 mmoles/dm3 in the soleus muscle. Pi was significantly lower in gastrocnemius (1.9 mmoles/dm3) than in soleus (3.2 mmoles/dm3). The ATP concentration was 6.7 and 6.4 mmoles/dm3 and pH was determined to 7.11 and 7.09 in the gastrocnemius and soleus muscle, respectively. Our NMR data show that it is possible to measure high-energy phosphates with precision in small localized volumes with the ISIS method using a Helmholtz coil. Earlier biochemical data are confirmed by these in vivo NMR results. Localized in vivo 31P NMR spectroscopy can contribute to the understanding of the underlying mechanisms of several metabolic events in different regions...Continue Reading

References

Jan 1, 1976·Biochemical Society Transactions·P J SeeleyR E Richards
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M J KushmerickR W Wiseman
Jul 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P R LuytenJ A den Hollander
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T R BrownK Ugurbil
Jan 1, 1993·Advances in Experimental Medicine and Biology·M J KushmerickR W Wiseman
Nov 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R Buchli, P Boesiger

❮ Previous
Next ❯

Citations

Jun 12, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·I R CorbinG Y Minuk
Feb 21, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Peter VermathenRoland Kreis
Feb 3, 1999·Annals of the New York Academy of Sciences·V WrayS Naruse
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Adam J Chicco, Genevieve C Sparagna
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S HitchinsG P Dobson
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J H Cieslar, G P Dobson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.