PMID: 36440Mar 1, 1979

Phosphorus NMR analysis of phospholipids in detergents

Journal of Lipid Research
E London, G W Feigenson

Abstract

Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. The resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents.

Related Concepts

In Vivo NMR Spectroscopy
Red Phosphorus
Sonication
Sarcoplasmic Reticulum
Cholic Acids
Phospholipids
Vigilon
Hydrogen-Ion Concentration
Synthetic Detergent

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