Phosphorus NMR and Its Application to Metabolomics.

Analytical Chemistry
Fatema BhinderwalaRobert Powers

Abstract

Stable isotopes are routinely employed by NMR metabolomics to highlight specific metabolic processes and to monitor pathway flux. 13C-carbon and 15N-nitrogen labeled nutrients are convenient sources of isotope tracers and are commonly added as supplements to a variety of biological systems ranging from cell cultures to animal models. Unlike 13C and 15N, 31P-phosphorus is a naturally abundant and NMR active isotope that does not require an external supplemental source. To date, 31P NMR has seen limited usage in metabolomics because of a lack of reference spectra, difficulties in sample preparation, and an absence of two-dimensional (2D) NMR experiments, but 31P NMR has the potential of expanding the coverage of the metabolome by detecting phosphorus-containing metabolites. Phosphorylated metabolites regulate key cellular processes, serve as a surrogate for intracellular pH conditions, and provide a measure of a cell's metabolic energy and redox state, among other processes. Thus, incorporating 31P NMR into a metabolomics investigation will enable the detection of these key cellular processes. To facilitate the application of 31P NMR in metabolomics, we present a unified protocol that allows for the simultaneous and efficient det...Continue Reading

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Citations

Sep 24, 2020·International Journal of Molecular Sciences·Aneta BalcerczykGilles J P Rautureau
Nov 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra A Crook, Robert Powers
Mar 13, 2021·Analytical Chemistry·Neil CoxGuy Lippens
May 13, 2021·Metabolomics : Official Journal of the Metabolomic Society·Biswapriya B Misra
Dec 8, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ganeko Bernardo-SeisdedosOscar Millet
Jul 14, 2021·Analytical and Bioanalytical Chemistry·Samuel FurseAlbert Koulman

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