PMID: 2110049Jan 1, 1990Paper

Phosphorylated and dephosphorylated myosin light chains of Drosophila fly and larva

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
H Takano-OhmuroK Maruyama

Abstract

1. The present study confirmed that light chains of Drosophila adult fibrillar (flight) muscle myosin consist of Lf1, Lf2, Lf2' and Lf3, and tubular muscle myosin light chains contain Lt1, Lt2, Lt2' and Lt3, as revealed by two-dimensional (isoelectric focusing and SDS-gel electrophoresis) gel electrophoresis. 2. Larva myosin light chains were of all the tubular type. However, it was found that Lt1 and Lt2' are produced by phosphorylation of Lt2, and Lf1 is produced by phosphorylation of Lf2'. 3. Injection of radioactive phosphate into Drosophila fly resulted in phosphorylations of Lf1 and Lt1. When larva or late pupa myosin was incubated with myosin light chain kinase from chicken gizzard or adult flies, phosphorylation of Lt1, Lf2' and Lt2' occurred. Drosophila myosin light chain kinase phosphorylated Lf1 in addition to Lt1 and L2' (Lf2' + Lt2') of adult myosin. 4. Dephosphorylation of adult myosin by potato acid and calf intestine alkaline phosphatases led to the shift of Lf1 (34,000), Lt1 (31,000) and L2' (Lf2' + Lt2') (30,000) to L2 (Lf2 + Lt2) positions (30,000). 5. Peptide mapping analyses revealed that larva Lt1, Lt2', Lt2 and adult Lt1 were all the same; therefore, it is thought that a single species of Lt2 specific to ...Continue Reading

References

Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S TakahashiK Maruyama
Jan 1, 1987·The Journal of Cell Biology·J ToffenettiM L Pardue
Aug 1, 1984·Journal of Biochemistry·S Nakamura, Y Nonomura
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Sep 1, 1983·Journal of Biochemistry·H Takano-OhmuroT Mikawa

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Citations

Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S TakahashiK Maruyama
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S TakahashiY Hotta
May 12, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Samantha PeronAram Megighian
Sep 11, 2002·Developmental Biology·You-Seung KimMark F A VanBerkum
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma
Nov 5, 2005·Journal of Muscle Research and Cell Motility·Sunita R Patel, Judith D Saide
Sep 5, 2003·American Journal of Physiology. Cell Physiology·Josh A HenkinJim O Vigoreaux

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