PMID: 9543497May 16, 1998Paper

Phosphorylated carboxy terminal serine residues stabilize the mouse gap junction protein connexin45 against degradation

The Journal of Membrane Biology
B HertleinO Traub

Abstract

Phosphoamino acid analysis of mouse connexin45 (Cx45) expressed in human HeLa cells revealed that phosphorylation occurred mainly at serine residues, but also on tyrosine and threonine residues. To characterize the role of Cx45 phosphorylation, different serine residues of the serine-rich carboxy terminal region were deleted or exchanged for other amino acids residues. Human HeLa cells deficient in gap junctional intercellular communication were stably transfected with appropriate constructs and analyzed for expression, localization, phosphorylation, formation of functional gap junction channels and degradation of mutant Cx45. fter exchange or deletion of nine carboxy terminal serine residues, phosphorylation was decreased by 90%, indicating that these serine residues represented main phosphorylation sites of mouse Cx45. The various serine residues of this region contributed differently to the phosphorylation of Cx45 suggesting a cooperative mechanism for phosphorylation. Substitution of different serine residues for other amino acids did not interfere with correct intracellular trafficking and assembly of functional gap junction channels, as shown by localization of mutant Cx45 at the plasma membrane and by dye transfer to nei...Continue Reading

Citations

May 23, 2001·Archives of Biochemistry and Biophysics·P D Lampe, A F Lau
Jun 29, 2012·The Journal of Membrane Biology·Scott R JohnstoneBrant E Isakson
Oct 14, 2003·Biochemical Pharmacology·Audrone KalvelyteFeliksas F Bukauskas
Feb 5, 2003·Biology of the Cell·Véronique Cruciani, Svein Ole Mikalsen
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Paul D Lampe, Alan F Lau
Jul 30, 2004·Molecular Biology of the Cell·Karen MaassKlaus Willecke
Dec 12, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Jennifer S FangKaren K Hirschi
Jun 22, 1999·The Journal of Clinical Investigation·M ChansonS Suter
Dec 13, 2006·Cell Communication & Adhesion·Philip R Gafken, Paul D Lampe
Dec 27, 2015·Seminars in Cell & Developmental Biology·Joell L Solan, Paul D Lampe
Aug 4, 2009·Experimental Eye Research·Zhen Wang, Kevin L Schey
Sep 29, 2006·The European Journal of Neuroscience·Karin DedekUlrike Janssen-Bienhold
May 31, 2013·Biology of the Cell·Catheleyne D'hondtGeert Bultynck
Feb 22, 2012·Biochimica Et Biophysica Acta·Jose F Ek-Vitorin, Janis M Burt
Mar 23, 2004·Biochimica Et Biophysica Acta·Bonnie J Warn-Cramer, Alan F Lau
Apr 14, 2000·Circulation Research·J E SaffitzK A Yamada
Sep 19, 1998·Circulation Research·D C Spray
Jun 26, 1999·Circulation Research·S AlcoléaD B Gros
Jan 11, 2003·Circulation Research·W Antoinette GroenewegenArthur A M Wilde
Feb 4, 1999·Journal of Cardiovascular Electrophysiology·X D HuangD P Zipes
Jan 26, 2006·The Journal of Comparative Neurology·Ruben S Van Der GiessenChris I De Zeeuw
Sep 22, 2017·Pharmacological Reviews·Luc LeybaertRainer Schulz
Sep 25, 2003·Physiological Reviews·Juan C SaezEric C Beyer
Mar 4, 2000·The Journal of Biological Chemistry·X YinJ X Jiang
Dec 2, 2020·Biomolecules·Joell L Solan, Paul D Lampe
Feb 15, 2000·Methods : a Companion to Methods in Enzymology·C D CooperP D Lampe
Feb 20, 2002·Brain Research·Chunbo Zhang, Diego Restrepo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.