Phosphorylated HSP27 modulates the association of phosphorylated caldesmon with tropomyosin in colonic smooth muscle

American Journal of Physiology. Gastrointestinal and Liver Physiology
Sita Somara, Khalil N Bitar

Abstract

Thin-filament regulation of smooth muscle contraction involves phosphorylation, association, and dissociation of contractile proteins in response to agonist stimulation. Phosphorylation of caldesmon weakens its association with actin leading to actomyosin interaction and contraction. Present data from colonic smooth muscle cells indicate that acetylcholine induced a significant association of caldesmon with PKCalpha and sustained phosphorylation of caldesmon at ser789. Furthermore, acetylcholine induced significant and sustained increase in the association of phospho-caldesmon with heat-shock protein (HSP)27 with concomitant increase in the dissociation of phospho-caldesmon from tropomyosin. At the thin filament level, HSP27 plays a crucial role in acetylcholine-induced association of contractile proteins. Present data from colonic smooth muscle cells transfected with non-phospho-HSP27 mutant cDNA indicate that the absence of phospho-HSP27 inhibits acetylcholine-induced caldesmon phosphorylation. Our results further indicate that the presence of phospho-HSP27 significantly enhances acetylcholine-induced sustained association of phospho-caldesmon with HSP27 with a concomitant increase in acetylcholine-induced dissociation of pho...Continue Reading

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Citations

Dec 17, 2009·Tissue Engineering. Part C, Methods·Shreya RaghavanKhalil N Bitar
May 27, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sita SomaraKhalil N Bitar
Sep 11, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sita SomaraKhalil N Bitar
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